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PABP cooperates with the CCR4-NOT complex to promote mRNA deadenylation and block precocious decay

DC Field Value Language
dc.contributor.authorYi, Hyerim-
dc.contributor.authorPark, Joha-
dc.contributor.authorHa, Minju-
dc.contributor.authorLim, Jaechul-
dc.contributor.authorChang, Hyeshik-
dc.contributor.authorKim, V. Narry-
dc.date.accessioned2021-01-31T08:15:25Z-
dc.date.available2021-01-31T08:15:25Z-
dc.date.created2019-06-21-
dc.date.created2019-06-21-
dc.date.issued2018-06-
dc.identifier.citationMolecular Cell, Vol.70 No.6, pp.1081-1088.e5-
dc.identifier.issn1097-2765-
dc.identifier.other76325-
dc.identifier.urihttps://hdl.handle.net/10371/171950-
dc.description.abstractMultiple deadenylases are known in vertebrates, the PAN2-PAN3 (PAN2/3) and CCR4-NOT (CNOT) complexes, and PARN, yet their differential functions remain ambiguous. Moreover, the role of poly(A) binding protein (PABP) is obscure, limiting our understanding of the deadenylation mechanism. Here, we show that CNOT serves as a predominant nonspecific deadenylase for cytoplasmic poly(A)+ RNAs, and PABP promotes deadenylation while preventing premature uridylation and decay. PAN2/3 selectively trims long tails (> similar to 150 nt) with minimal effect on transcriptome, whereas PARN does not affect mRNA deadenylation. CAF1 and CCR4, catalytic subunits of CNOT, display distinct activities: CAF1 trims naked poly(A) segments and is blocked by PABPC, whereas CCR4 is activated by PABPC to shorten PABPC-protected sequences. Concerted actions of CAF1 and CCR4 delineate the similar to 27 nt periodic PABPC footprints along shortening tail. Our study unveils distinct functions of deadenylases and PABPC, re-drawing the view on mRNA deadenylation and regulation.-
dc.language영어-
dc.publisherCell Press-
dc.titlePABP cooperates with the CCR4-NOT complex to promote mRNA deadenylation and block precocious decay-
dc.typeArticle-
dc.contributor.AlternativeAuthor김빛내리-
dc.identifier.doi10.1016/j.molcel.2018.05.009-
dc.citation.journaltitleMolecular Cell-
dc.identifier.wosid000436640300012-
dc.identifier.scopusid2-s2.0-85048205542-
dc.citation.endpage1088.e5-
dc.citation.number6-
dc.citation.startpage1081-
dc.citation.volume70-
dc.identifier.sci000436640300012-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorLim, Jaechul-
dc.contributor.affiliatedAuthorChang, Hyeshik-
dc.contributor.affiliatedAuthorKim, V. Narry-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusPOLY(A) BINDING-PROTEIN-
dc.subject.keywordPlusPOLY(A)-BINDING PROTEIN-
dc.subject.keywordPlusTRANSLATIONAL REPRESSION-
dc.subject.keywordPlusTRANSCRIPTION FACTOR-
dc.subject.keywordPlusCAF1 PROTEINS-
dc.subject.keywordPlusTAIL LENGTH-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusTERMINATION-
dc.subject.keywordPlusSTABILITY-
dc.subject.keywordPlusMECHANISM-
dc.subject.keywordAuthorCAF1-
dc.subject.keywordAuthorCCR4-
dc.subject.keywordAuthorCCR4-NOT-
dc.subject.keywordAuthordeadenylation-
dc.subject.keywordAuthorPABPC-
dc.subject.keywordAuthorPAN2-PAN3-
dc.subject.keywordAuthorPARN-
dc.subject.keywordAuthorpoly(A) tail-
dc.subject.keywordAuthorRNA decay-
dc.subject.keywordAuthoruridylation-
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  • School of Biological Sciences
Research Area Molecular Biology & Genetics

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