Browse

Terminal uridylyltransferases execute programmed clearance of maternal transcriptome in vertebrate embryos

Cited 28 time in Web of Science Cited 26 time in Scopus
Authors
Chang, Hyeshik; Yeo, Jinah; Kim, Jeong-gyun; Kim, Hyunjoon; Lim, Jaechul; Lee, Mihye; Kim, Hyun Ho; Ohk, Jiyeon; Jeon, Hee-Yeon; Lee, Hyunsook; Jung, Hosung; Kim, Kyu-Won; Kim, V. Narry
Issue Date
2018-04
Citation
Molecular Cell, Vol.70 No.1, pp.72-82.e7
Abstract
During the maternal-to-zygotic transition (MZT), maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genome-wide profiling of RNA abundance and 30 modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development.
ISSN
1097-2765
URI
https://hdl.handle.net/10371/171954
DOI
https://doi.org/10.1016/j.molcel.2018.03.004
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse