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SHORT VEGETATIVE PHASE (SVP) protein negatively regulates miR172 transcription via direct binding to the pri-miR172a promoter in Arabidopsis

Cited 37 time in Web of Science Cited 41 time in Scopus
Authors
Cho, Hyun Jung; Kim, Jae Joon; Lee, Jeong Hwan; Kim, Wanhui; Jung, Jae-Hoon; Park, Chung-Mo; Ahn, Ji Hoon
Issue Date
2012-07
Citation
FEBS Letters, Vol.586 No.16, pp.2332-2337
Keywords
SVPmiR172Pri-miR172aTranscriptionFlowering time
Abstract
Plant microRNAs (miRNAs) are non-coding RNAs that negatively regulate expression of their target genes. Although much is known about miRNA biogenesis and repression of target genes by miRNAs, the molecular mechanisms underlying the transcriptional regulation of miRNA itself are poorly understood. Here, we report that SHORT VEGETATIVE PHASE (SVP) protein is a direct transcriptional regulator of miR172. The levels of mature miR172 and pri-miR172a were anti-correlated with SVP activity. miR172a has multiple transcription start sites, among which the transcript starting with cytosine (-671 bp, relative to the mature miR172a) was a major species. EMSA and ChIP analysis demonstrated that SVP protein binds to the CArG motifs in the miR172a promoter. These results suggest that SVP protein directly regulates miR172 transcription in Arabidopsis. Crown Copyright (C) 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.
ISSN
0014-5793
URI
https://hdl.handle.net/10371/171984
DOI
https://doi.org/10.1016/j.febslet.2012.05.035
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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