Browse

Root-expressed phytochromes B1 and B2, but not PhyA and Cry2, regulate shoot growth in nature

Cited 2 time in Web of Science Cited 2 time in Scopus
Authors
Oh, Youngjoo; Fragoso, Variluska; Guzzonato, Francesco; Kim, Sang-Gyu; Park, Chung-Mo; Baldwin, Ian T.
Issue Date
2018-11
Citation
Plant, Cell and Environment, Vol.41 No.11, pp.2577-2588
Keywords
graftinggrowthHY5lightphytochrome Broots
Abstract
Although photoreceptors are expressed throughout all plant organs, most studies have focused on their function in aerial parts with laboratory-grown plants. Photoreceptor function in naturally dark-grown roots of plants in their native habitats is lacking. We characterized patterns of photoreceptor expression in field- and glasshouse-grown Nicotiana attenuata plants, silenced the expression of PhyB1/B2/A/Cry2 whose root transcripts levels were greater/equal to those of shoots, and by micrografting combined empty vector transformed shoots onto photoreceptor-silenced roots, creating chimeric plants with "blind" roots but "sighted" shoots. Micrografting procedure was robust in both field and glasshouse, as demonstrated by transcript accumulation patterns, and a spatially-explicit lignin visual reporter chimeric line. Field- and glasshouse-grown plants with PhyB1B2, but not PhyA or Cry2, -blind roots, were delayed in stalk elongation compared with control plants, robustly for two field seasons. Wild-type plants with roots directly exposed to FR phenocopied the growth of irPhyB1B2-blind root grafts. Additionally, root-expressed PhyB1B2 was required to activate the positive photomorphogenic regulator, HY5, in response to aboveground light. We conclude that roots of plants growing deep into the soil in nature sense aboveground light, and possibly soil temperature, via PhyB1B2 to control key traits, such as stalk elongation.
ISSN
0140-7791
URI
https://hdl.handle.net/10371/172087
DOI
https://doi.org/10.1111/pce.13341
Files in This Item:
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse