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3D graphene-cellulose nanofiber hybrid scaffolds for cortical reconstruction in brain injuries

Cited 4 time in Web of Science Cited 7 time in Scopus
Issue Date
2019-10
Citation
2D Materials, Vol.6 No.4, p. 045043
Keywords
graphene oxidebacterial cellulosecortex-mimetic 3D hybrid scaffoldcorticectomized ratphotoacoustic
Abstract
Designing the nature-driven 3D scaffold is essential for reconstructing of the injured brain in association with stem cell replacement therapy. In this paper, we developed brain cortex-mimetic 3D hybrid scaffolds and applied them to a motor-cortectomy rat model. Graphene oxide bacterial cellulose (GO-BC) hybrid scaffold integrated GOs stably and homogeneously within BC nanofibrous building blocks made of BC and amphiphilic comb-like polymers (APCLP). Density functional theory calculations and molecular dynamics simulations revealed higher binding energies between GO-BC and APCLP than between GO or APLCP with BC. The monodispersed human neural stem cells (F3 cells) incorporated within the GO-BC scaffold generated a large number of differentiated neurons with robust neurite outgrowths and possible synapse formation in vitro. In corticectomized rats and nude mice, highly sensitive photoacoustic signals visualized the GO-BC at the implant site. Moreover, the implanted F3 cells within GO-BC were found to survive/proliferate and differentiate to neuronal lineage from the showing neuronal and synapse markers shown on ex vivo immunofluorescence staining in bioluminescence imaging. Cortex-mimetic and stem cell-instructive monodisperse GO-BC hybrid scaffolds are likely to be appropriate nanoplatforms for stem cell implantation to reconstruct injured/lost brain tissues and actively differentiate neural stem cells.
ISSN
2053-1583
URI
https://hdl.handle.net/10371/172113
DOI
https://doi.org/10.1088/2053-1583/ab3889
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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