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Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy
Cited 66 time in
Web of Science
Cited 68 time in Scopus
- Authors
- Issue Date
- 2009-08
- Publisher
- Academic Press
- Citation
- Analytical Biochemistry, Vol.391 No.1, pp.24-30
- Abstract
- This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved.
- ISSN
- 0003-2697
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