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Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy

Cited 59 time in Web of Science Cited 63 time in Scopus
Authors
Jun, Bong-Hyun; Noh, Mi Suk; Kim, Gunsung; Kang, Homan; Kim, Jong-Ho; Chung, Woo-Jae; Kim, Min-Soo; Kim, Yong-Kweon; Cho, Myung-Haing; Jeong, Dae Hong; Lee, Yoon-Sik
Issue Date
2009-08
Citation
Analytical Biochemistry, Vol.391 No.1, pp.24-30
Keywords
Silver nanoparticlesOptically active materialsMagnetic materialsPolymeric materialsSurface-enhanced Raman scattering
Abstract
This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
0003-2697
URI
https://hdl.handle.net/10371/172294
DOI
https://doi.org/10.1016/j.ab.2009.05.005
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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