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Chitosan-graft-spermine as a gene carrier in vitro and in vivo

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dc.contributor.authorJiang, Hu-Lin-
dc.contributor.authorLim, Hwang-Tae-
dc.contributor.authorKim, You-Kyoung-
dc.contributor.authorArote, Rohidas-
dc.contributor.authorShin, Ji-Young-
dc.contributor.authorKwon, Jung-Taek-
dc.contributor.authorKim, Ji-Eun-
dc.contributor.authorKim, Ji-Hye-
dc.contributor.authorKim, Duyeol-
dc.contributor.authorChae, Chanhee-
dc.contributor.authorNah, Jae-Woon-
dc.contributor.authorChoi, Yun-Jaie-
dc.contributor.authorCho, Chong-Su-
dc.contributor.authorCho, Myung-Haing-
dc.date.accessioned2021-01-31T08:42:10Z-
dc.date.available2021-01-31T08:42:10Z-
dc.date.issued2011-01-
dc.identifier.citationEUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, Vol.77 No.1, pp.36-42-
dc.identifier.issn0939-6411-
dc.identifier.other14255-
dc.identifier.urihttps://hdl.handle.net/10371/172372-
dc.description.abstractChitosan has been proposed as a non-viral gene carrier because of its biodegradable and biocompatible cationic polymeric properties. However, the transfection efficiency of chitosan-DNA complexes is still too low for clinical trials. To improve transfection efficiency, we prepared a chitosan-graft-spermine (CHI-g-SPE) copolymer by an imine reaction between periodate-oxidized chitosan and spermine. The CHI-g-SPE copolymer was complexed with plasmid DNA in various copolymer-DNA weight ratios, and the complexes were characterized. The CHI-g-SPE copolymer showed good DNA binding ability and high protection of DNA from nuclease attack. The CHI-g-SPE/DNA complexes had well-formed spherical shapes and a nanoscale size with homogenous size distribution. The CHI-g-SPE copolymer had low cytotoxicity and CHI-g-SPE/DNA complexes showed transfection efficiency that was enhanced over that of chitosan-DNA. Furthermore, aerosol delivery of CHI-g-SPE/GFP complexes showed higher GFP expression compared with chitosan/GFP complexes, without toxicity. Our results indicate that the CHI-g-SPE copolymer has potential as a gene carrier. (C) 2010 Elsevier B.V. All rights reserved.-
dc.subjectGene therapy-
dc.subjectNon-viral gene delivery-
dc.subjectChitosan-
dc.subjectSpermine-
dc.subjectCytotoxicity-
dc.subjectAerosol delivery-
dc.titleChitosan-graft-spermine as a gene carrier in vitro and in vivo-
dc.typeArticle-
dc.contributor.AlternativeAuthor조명행-
dc.identifier.doi10.1016/j.ejpb.2010.09.014-
dc.citation.journaltitleEUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS-
dc.identifier.scopusid2-s2.0-78650695293-
dc.citation.endpage42-
dc.citation.number1-
dc.citation.startpage36-
dc.citation.volume77-
dc.identifier.rimsid14255-
dc.identifier.sci000286687500006-
dc.contributor.affiliatedAuthorCho, Myung-Haing-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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