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Chitosan-graft-spermine as a gene carrier in vitro and in vivo
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Hu-Lin | - |
dc.contributor.author | Lim, Hwang-Tae | - |
dc.contributor.author | Kim, You-Kyoung | - |
dc.contributor.author | Arote, Rohidas | - |
dc.contributor.author | Shin, Ji-Young | - |
dc.contributor.author | Kwon, Jung-Taek | - |
dc.contributor.author | Kim, Ji-Eun | - |
dc.contributor.author | Kim, Ji-Hye | - |
dc.contributor.author | Kim, Duyeol | - |
dc.contributor.author | Chae, Chanhee | - |
dc.contributor.author | Nah, Jae-Woon | - |
dc.contributor.author | Choi, Yun-Jaie | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2021-01-31T08:42:10Z | - |
dc.date.available | 2021-01-31T08:42:10Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, Vol.77 No.1, pp.36-42 | - |
dc.identifier.issn | 0939-6411 | - |
dc.identifier.other | 14255 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172372 | - |
dc.description.abstract | Chitosan has been proposed as a non-viral gene carrier because of its biodegradable and biocompatible cationic polymeric properties. However, the transfection efficiency of chitosan-DNA complexes is still too low for clinical trials. To improve transfection efficiency, we prepared a chitosan-graft-spermine (CHI-g-SPE) copolymer by an imine reaction between periodate-oxidized chitosan and spermine. The CHI-g-SPE copolymer was complexed with plasmid DNA in various copolymer-DNA weight ratios, and the complexes were characterized. The CHI-g-SPE copolymer showed good DNA binding ability and high protection of DNA from nuclease attack. The CHI-g-SPE/DNA complexes had well-formed spherical shapes and a nanoscale size with homogenous size distribution. The CHI-g-SPE copolymer had low cytotoxicity and CHI-g-SPE/DNA complexes showed transfection efficiency that was enhanced over that of chitosan-DNA. Furthermore, aerosol delivery of CHI-g-SPE/GFP complexes showed higher GFP expression compared with chitosan/GFP complexes, without toxicity. Our results indicate that the CHI-g-SPE copolymer has potential as a gene carrier. (C) 2010 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | LOW-MOLECULAR-WEIGHT | - |
dc.subject | TRANSFECTION EFFICIENCY | - |
dc.subject | DELIVERY-SYSTEM | - |
dc.subject | NONVIRAL VECTOR | - |
dc.subject | DNA DELIVERY | - |
dc.subject | GLYCOL) COPOLYMERS | - |
dc.subject | LUNG-CANCER | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | THERAPY | - |
dc.subject | WATER | - |
dc.title | Chitosan-graft-spermine as a gene carrier in vitro and in vivo | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1016/j.ejpb.2010.09.014 | - |
dc.citation.journaltitle | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | - |
dc.identifier.wosid | 000286687500006 | - |
dc.identifier.scopusid | 2-s2.0-78650695293 | - |
dc.citation.endpage | 42 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 36 | - |
dc.citation.volume | 77 | - |
dc.identifier.sci | 000286687500006 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Chae, Chanhee | - |
dc.contributor.affiliatedAuthor | Choi, Yun-Jaie | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | DELIVERY-SYSTEM | - |
dc.subject.keywordPlus | NONVIRAL VECTOR | - |
dc.subject.keywordPlus | DNA DELIVERY | - |
dc.subject.keywordPlus | GLYCOL) COPOLYMERS | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordAuthor | Gene therapy | - |
dc.subject.keywordAuthor | Non-viral gene delivery | - |
dc.subject.keywordAuthor | Chitosan | - |
dc.subject.keywordAuthor | Spermine | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Aerosol delivery | - |
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