Carnosine and related compounds protect against copper-induced damage of biomolecules

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Lee, Beom Jun; Lee, Yong Soon; Kang, Kyung Sun; Cho, Myung-Haing; Hendricks, Deloy G.

Issue Date
Springer Verlag
Journal of Biochemistry and Molecular Biology, Vol.32 No.4, pp.350-357
At concentrations of 1 mM, the protective effects of carnosine and related compounds including anserine, homocarnosine, histidine, and beta-alanine were investigated against copper-catalyzed oxidative damage to deoxyribose, ascorbic acid, human serum albumin, liposome, and erythrocytes. Carnosine and anserine reduced Cu (II) to bathocuproine-reactive Cu (I) in a time- and a dose-dependent manner while the others did not. Carnosine reduced 86% of 100 mu M CU (TI) in 60 min. Carnosine, homocarnosine, anserine, and histidine inhibited copper-catalyzed deoxyribose degradation by 75, 66, 65, and 45%, respectively. In the presence of 1 mu M Cu (II), carnosine and related compounds inhibited ascorbic acid oxidation by 55-85% after incubation for 20 min. In the presence of 0.15 mM ascorbic acid and 0.8 mM H2O2 carnosine, anserine, homocarnosine, and histidine inhibited copper-catalyzed oxidation of human serum albumin by 41, 21, 29, and 24%, respectively, as determined by carbonyl formation. These compounds also significantly inhibited copper-catalyzed liposomal lipid peroxidation as measured by malondialdehyde and lipid hydroperoxides. Carnosine, anserine, homocarnosine, and histidine inhibited hemolysis of bovine erythrocytes induced by 0.1 mM Cu (II), These results suggest that histidine-containing dipeptides may play an important role in protecting against free radical-mediated tissue damage.
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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