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Galactosylation of Chitosan-Graft-Spermine as a Gene Carrier for Hepatocyte Targeting In Vitro and In Vivo
DC Field | Value | Language |
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dc.contributor.author | Kim, Ji-Hye | - |
dc.contributor.author | Kim, You-Kyoung | - |
dc.contributor.author | Arash, Minai-Tehrani | - |
dc.contributor.author | Hong, Seong-Ho | - |
dc.contributor.author | Lee, Jae-Ho | - |
dc.contributor.author | Kang, Bit Na | - |
dc.contributor.author | Bang, Yong-Bin | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.contributor.author | Yu, Dae-Yeul | - |
dc.contributor.author | Jiang, Hu-Lin | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2021-01-31T08:45:51Z | - |
dc.date.available | 2021-01-31T08:45:51Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.citation | Journal of Nanoscience and Nanotechnology, Vol.12 No.7, pp.5178-5184 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.other | 13271 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172434 | - |
dc.description.abstract | Polyethyleneimine (PEI) has been described as a highly efficient gene carrier due to its efficient proton sponge effect within endosomes. However, many studies have demonstrated that PEI is toxic and associated with a lack of cell specificity despite high transfection efficiency. In order to minimize the toxicity of PEI, we prepared chitosan-graft-spermine (CHI-g-SPE) in a previous study. CHI-g-SPE showed low toxicity and high transfection efficiency. However, this compound also had limited target cell specificity. In the present study, we synthesized galactosylated CHI-g-SPE (GCS) because this modified GCS could be delivered specifically into the liver due to hepatocyte-specific galactose receptors. The DNA-binding properties of GCS at various copolymer/DNA weight ratios were evaluated by a gel retardation assay. The GCS copolymer exhibited significant DNA-binding ability and efficiently protected DNA from nuclease attack. Using energy-filtered transmission electron microscopy (EF-TEM), we observed dense spherical, nano-sized GCS/DNA complexes with a homogenous distribution. Most importantly, GCS was associated with remarkably low cytotoxicity compared to PEI in HepG2, HeLa, and A549 cells. Moreover, GCS carriers specifically delivered the gene-of-interest into hepatocytes in vitro as well as in vivo. Our results suggest that the novel GCS described here is a safe and highly efficient carrier for hepatocyte-targeted gene delivery. | - |
dc.language | 영어 | - |
dc.publisher | American Scientific Publishers | - |
dc.title | Galactosylation of Chitosan-Graft-Spermine as a Gene Carrier for Hepatocyte Targeting In Vitro and In Vivo | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1166/jnn.2012.6376 | - |
dc.citation.journaltitle | Journal of Nanoscience and Nanotechnology | - |
dc.identifier.wosid | 000307604700011 | - |
dc.identifier.scopusid | 2-s2.0-84865133579 | - |
dc.citation.endpage | 5184 | - |
dc.citation.number | 7 | - |
dc.citation.startpage | 5178 | - |
dc.citation.volume | 12 | - |
dc.identifier.sci | 000307604700011 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | DNA NANOPARTICLES | - |
dc.subject.keywordPlus | NONVIRAL VECTOR | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Gene Therapy | - |
dc.subject.keywordAuthor | Non-Viral Gene Delivery | - |
dc.subject.keywordAuthor | Hepatocyte Targeting | - |
dc.subject.keywordAuthor | Galactosylated Chitosan-Graft-Spermine | - |
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