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Immobilization of Aptamer-Based Molecular Beacons Onto Optically-Encoded Micro-Sized Beads

Cited 9 time in Web of Science Cited 9 time in Scopus
Authors
Jun, Bong-Hyun; Kim, Ji-Eun; Rho, Chul; Byun, Jang-Woong; Kim, Yo Han; Kang, Homan; Kim, Jong-Ho; Kang, Taegyu; Cho, Myung-Haing; Lee, Yoon-Sik
Issue Date
2011-07
Citation
Journal of Nanoscience and Nanotechnology, Vol.11 No.7, pp.6249-6252
Keywords
Molecular BeaconEncoded BeadThrombin DetectionHigh-Throughput Screening (HTS)
Abstract
This paper presents a method for the novel immobilization of aptamer-based molecular beacons (apta-beacons) onto optically-encoded micro-sized beads (apta-beacon beads). To immobilize apta-beacons onto flourescently-encoded micro-sized beads, core shell type beads containing a fluorescent dye-encoded core and apta beacon-coupled shell were prepared. The fluorescent dye-encoded beads were prepared from TentaGel resins by coupling RITC to the amino groups of the core region, after partial protection of amino groups with Fmoc-OSu in a diffusion-controlled manner. After deprotection of the Fmoc-amino groups, FITC-coupled molecular beacons (MBs) were immobilized to the beads together with a quencher by covelent bonding. Briefly, aspartic acid (Asp) was coupled to the shell part of the beads. Then, the quencher was coupled to the N-terminal amino group of Asp and the MBs were coupled to the side chain carboxyl group. In a model study, thrombin was directly detected using this apta-beacon bead method. The thrombin-bound apta-beacon beads were easily recognized by the appearance of fluorescence without any further labeling step.
ISSN
1533-4880
URI
https://hdl.handle.net/10371/172439
DOI
https://doi.org/10.1166/jnn.2011.4501
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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