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Immobilization of Aptamer-Based Molecular Beacons Onto Optically-Encoded Micro-Sized Beads
DC Field | Value | Language |
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dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Kim, Ji-Eun | - |
dc.contributor.author | Rho, Chul | - |
dc.contributor.author | Byun, Jang-Woong | - |
dc.contributor.author | Kim, Yo Han | - |
dc.contributor.author | Kang, Homan | - |
dc.contributor.author | Kim, Jong-Ho | - |
dc.contributor.author | Kang, Taegyu | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.date.accessioned | 2021-01-31T08:46:16Z | - |
dc.date.available | 2021-01-31T08:46:16Z | - |
dc.date.created | 2020-12-15 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.citation | Journal of Nanoscience and Nanotechnology, Vol.11 No.7, pp.6249-6252 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.other | 119152 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172439 | - |
dc.description.abstract | This paper presents a method for the novel immobilization of aptamer-based molecular beacons (apta-beacons) onto optically-encoded micro-sized beads (apta-beacon beads). To immobilize apta-beacons onto flourescently-encoded micro-sized beads, core shell type beads containing a fluorescent dye-encoded core and apta beacon-coupled shell were prepared. The fluorescent dye-encoded beads were prepared from TentaGel resins by coupling RITC to the amino groups of the core region, after partial protection of amino groups with Fmoc-OSu in a diffusion-controlled manner. After deprotection of the Fmoc-amino groups, FITC-coupled molecular beacons (MBs) were immobilized to the beads together with a quencher by covelent bonding. Briefly, aspartic acid (Asp) was coupled to the shell part of the beads. Then, the quencher was coupled to the N-terminal amino group of Asp and the MBs were coupled to the side chain carboxyl group. In a model study, thrombin was directly detected using this apta-beacon bead method. The thrombin-bound apta-beacon beads were easily recognized by the appearance of fluorescence without any further labeling step. | - |
dc.language | 영어 | - |
dc.publisher | American Scientific Publishers | - |
dc.title | Immobilization of Aptamer-Based Molecular Beacons Onto Optically-Encoded Micro-Sized Beads | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1166/jnn.2011.4501 | - |
dc.citation.journaltitle | Journal of Nanoscience and Nanotechnology | - |
dc.identifier.wosid | 000293663200120 | - |
dc.identifier.scopusid | 2-s2.0-84855398927 | - |
dc.citation.endpage | 6252 | - |
dc.citation.number | 7 | - |
dc.citation.startpage | 6249 | - |
dc.citation.volume | 11 | - |
dc.identifier.sci | 000293663200120 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.contributor.affiliatedAuthor | Lee, Yoon-Sik | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | LIBRARY | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | SERS | - |
dc.subject.keywordAuthor | Molecular Beacon | - |
dc.subject.keywordAuthor | Encoded Bead | - |
dc.subject.keywordAuthor | Thrombin Detection | - |
dc.subject.keywordAuthor | High-Throughput Screening (HTS) | - |
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