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Immobilization of Aptamer-Based Molecular Beacons Onto Optically-Encoded Micro-Sized Beads

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dc.contributor.authorJun, Bong-Hyun-
dc.contributor.authorKim, Ji-Eun-
dc.contributor.authorRho, Chul-
dc.contributor.authorByun, Jang-Woong-
dc.contributor.authorKim, Yo Han-
dc.contributor.authorKang, Homan-
dc.contributor.authorKim, Jong-Ho-
dc.contributor.authorKang, Taegyu-
dc.contributor.authorCho, Myung-Haing-
dc.contributor.authorLee, Yoon-Sik-
dc.date.accessioned2021-01-31T08:46:16Z-
dc.date.available2021-01-31T08:46:16Z-
dc.date.created2020-12-15-
dc.date.issued2011-07-
dc.identifier.citationJournal of Nanoscience and Nanotechnology, Vol.11 No.7, pp.6249-6252-
dc.identifier.issn1533-4880-
dc.identifier.other119152-
dc.identifier.urihttps://hdl.handle.net/10371/172439-
dc.description.abstractThis paper presents a method for the novel immobilization of aptamer-based molecular beacons (apta-beacons) onto optically-encoded micro-sized beads (apta-beacon beads). To immobilize apta-beacons onto flourescently-encoded micro-sized beads, core shell type beads containing a fluorescent dye-encoded core and apta beacon-coupled shell were prepared. The fluorescent dye-encoded beads were prepared from TentaGel resins by coupling RITC to the amino groups of the core region, after partial protection of amino groups with Fmoc-OSu in a diffusion-controlled manner. After deprotection of the Fmoc-amino groups, FITC-coupled molecular beacons (MBs) were immobilized to the beads together with a quencher by covelent bonding. Briefly, aspartic acid (Asp) was coupled to the shell part of the beads. Then, the quencher was coupled to the N-terminal amino group of Asp and the MBs were coupled to the side chain carboxyl group. In a model study, thrombin was directly detected using this apta-beacon bead method. The thrombin-bound apta-beacon beads were easily recognized by the appearance of fluorescence without any further labeling step.-
dc.language영어-
dc.publisherAmerican Scientific Publishers-
dc.titleImmobilization of Aptamer-Based Molecular Beacons Onto Optically-Encoded Micro-Sized Beads-
dc.typeArticle-
dc.contributor.AlternativeAuthor조명행-
dc.identifier.doi10.1166/jnn.2011.4501-
dc.citation.journaltitleJournal of Nanoscience and Nanotechnology-
dc.identifier.wosid000293663200120-
dc.identifier.scopusid2-s2.0-84855398927-
dc.citation.endpage6252-
dc.citation.number7-
dc.citation.startpage6249-
dc.citation.volume11-
dc.identifier.sci000293663200120-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorCho, Myung-Haing-
dc.contributor.affiliatedAuthorLee, Yoon-Sik-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusLIBRARY-
dc.subject.keywordPlusPROBES-
dc.subject.keywordPlusSERS-
dc.subject.keywordAuthorMolecular Beacon-
dc.subject.keywordAuthorEncoded Bead-
dc.subject.keywordAuthorThrombin Detection-
dc.subject.keywordAuthorHigh-Throughput Screening (HTS)-
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