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Lentiviral vector-mediated shRNA against AIMP2-DX2 suppresses lung cancer cell growth through blocking glucose uptake
DC Field | Value | Language |
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dc.contributor.author | Chang, Seung-Hee | - |
dc.contributor.author | Chung, Youn-Sun | - |
dc.contributor.author | Hwang, Soon-Kyung | - |
dc.contributor.author | Kwon, Jung-Taek | - |
dc.contributor.author | Minai-Tehrani, Arash | - |
dc.contributor.author | Kim, Sunghoon | - |
dc.contributor.author | Park, Seung Bum | - |
dc.contributor.author | Kim, Yeon-Soo | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2021-01-31T08:48:22Z | - |
dc.date.available | 2021-01-31T08:48:22Z | - |
dc.date.created | 2020-11-16 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.citation | Molecules and Cells, Vol.33 No.6, pp.553-562 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 116330 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172476 | - |
dc.description.abstract | Aminoacyl-tRNA synthetases [ARS]-interacting multifunctional protein 2 (AIMP2) has been implicated in the control of cell fate and lung cell differentiation. A variant of AIMP2 lacking exon 2 (AIMP2-DX2) is expressed in different cancer cells. We previously studied the expression level of AIMP2-DX2 in several lung cell lines and reported elevated expression levels of AIMP2-DX2 in NCI-H460 and NCI-H520. Here, we report that the suppression of AIMP2-DX2 by lentivirus mediated short hairpin (sh)RNA (sh-DX2) decreased the rate of glucose uptake and glucose transporters (Gluts) in NCI-H460 cells. Down-regulation of AIMP2-DX2 reduced glycosyltransferase (GnT)-V in the Golgi apparatus, while inducing the GnT-V antagonist GnT-III. Down-regulation of AIMP2-DX2 also suppressed the epidermal growth factor receptor/mitogen activated protein kinase (EGFR/MAPK) signaling pathway, leading to the decrease of the proliferation marker Ki-67 expression in nuclei. Furthermore, dual luciferase activity reduced capdependent protein translation in cells infected with sh-DX2. These results suggest that AIMP2-DX2 may be a relevant therapeutic target for lung cancer, and that the sh-DX2 lentiviral system can be an appropriate method for lung cancer therapy. | - |
dc.language | 영어 | - |
dc.publisher | 한국분자세포생물학회 | - |
dc.title | Lentiviral vector-mediated shRNA against AIMP2-DX2 suppresses lung cancer cell growth through blocking glucose uptake | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1007/s10059-012-2269-2 | - |
dc.citation.journaltitle | Molecules and Cells | - |
dc.identifier.wosid | 000305691100004 | - |
dc.identifier.scopusid | 2-s2.0-84863486837 | - |
dc.citation.endpage | 562 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 553 | - |
dc.citation.volume | 33 | - |
dc.identifier.sci | 000305691100004 | - |
dc.identifier.kciid | ART001668150 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Seung Bum | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | N-ACETYLGLUCOSAMINYLTRANSFERASE-V | - |
dc.subject.keywordPlus | RNA SYNTHETASE COFACTOR | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI-CELLS | - |
dc.subject.keywordPlus | BETA-1,6-BRANCHED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | C-MYC | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | TRANSPORTER | - |
dc.subject.keywordPlus | STAGE | - |
dc.subject.keywordAuthor | AIMP2-DX2 | - |
dc.subject.keywordAuthor | EGFR/MAPK signaling pathway | - |
dc.subject.keywordAuthor | glucose uptake | - |
dc.subject.keywordAuthor | gluts | - |
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