Browse

Toxic response of HIPCO single-walled carbon nanotubes in mice and RAW264.7 macrophage cells

Cited 19 time in Web of Science Cited 18 time in Scopus
Authors
Park, Eun-Jung; Zahari, Nur Elida M.; Kang, Min-Sung; Lee, Sang Jin; Lee, Kyuhong; Lee, Byoung-Seok; Yoon, Cheolho; Cho, Myung-Haing; Kim, Younghun; Kim, Jae-Ho
Issue Date
2014-08
Citation
Toxicology Letters, Vol.229 No.1, pp.167-177
Keywords
SWCNTMacrophagesApoptosisAutophagyMitochondriaER stress
Abstract
In this study, we identified the toxic response of pristine single-walled carbon nanotubes (P-SWCNTs) synthesized by HIPCO method in mice and RAW264.7 cells, a murine peritoneal macrophage cell line. P-SWCNT contained a large amount of Fe ion (36 wt%). In the lungs of mice 24 h after intratracheal administration, P-SWCNTs increased the secretion of IL-6 and MCP-1, and the number of total cells, the portion of neutrophils, lymphocytes, and eosinophils, also significantly increased at a 100 mg/mL of concentration. In RAW264.7 cells, cell viability and ATP production decreased in a dose-dependent manner at 24 h after exposure, whereas the generations of ROS and NO were enhanced at all concentrations together with the activation of the MAP kinase pathway. Moreover, the levels of both apoptosis-and autophagy-related proteins and ER stress-related proteins clearly increased, and the concentrations of Fe, Cu, and Zn ions, but not of Mn ions, increased in a dose-dependent manner. TEM images also revealed that P-SWCNTs induced the formation of autophagosome-like vacuoles, the dilatation of the ER, the generation of mitochondrial flocculent densities, and the separation of organelle by disappearance of the cell membrane. Taken together, we suggest that P-SWCNTs cause acute inflammatory response in the lungs of mice, and induce autophagy accompanied with apoptosis through mitochondrial dysfunction and ER stress in RAW264.7 cells. Furthermore, further study is required to elucidate how the physicochemical properties of SWCNTs determine the cell death pathway and an immune response. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
ISSN
0378-4274
URI
https://hdl.handle.net/10371/172528
DOI
https://doi.org/10.1016/j.toxlet.2014.06.015
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse