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Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol

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Authors
Kim, Areumnuri; Kim, Joong-Young; Jeong, Sang-Hee; Cho, Myung-Haing; Park, Kyung-Hun; Cho, Namjun; Paik, Min Kyoung
Issue Date
2015-12
Citation
한국환경농학회지, Vol.34 No.4, pp.345-349
Keywords
ChlorpyrifosHaptenTCP
Abstract
BACKGROUND: In this study, we have attempted to identify a urinary biomarker to assess chlorpyrifos exposure in farmers. The major metabolite and the excretion pathway of chlorpyrifos is 3,5,6-trichloro-2- pyridinol (TCP) in urine. Herein, we describe an adequate synthetic method for TCP hapten for measuring urinary TCP of farmers. METHODS AND RESULTS: First, TCP was prepared by spacer attachment through hydrolysis of thiophosphate ester from chlorpyrifos. After reaction with benzyl bromide, the TCP was transformed into 2,3,5-trichloro-6- benzyloxypyridine. Next, the chlorine in the 2nd position of the pyridyl ring was substituted into 3-mercaptopropanoic acid spacer arm. Finally, the phenyl group attached to the 6th position in pyridyl ring was removed for producing the targeted product, 3-(3,5-Dichloro-6-hydroxy-2-pyridyl) thiopropanoic acid. CONCLUSION: Henceforth, this TCP hapten would be used in developing immunoassay studies for the detection and quantitation of urinary TCP of farmers.
ISSN
1225-3537
URI
https://hdl.handle.net/10371/172544
DOI
https://doi.org/10.5338/KJEA.2015.34.4.38
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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