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15-Keto prostaglandin E2 induces heme oxygenase-1 expression through activation of Nrf2 in human colon epithelial CCD 841 CoN cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong-Eun | - |
dc.contributor.author | Zhong, Xiancai | - |
dc.contributor.author | Lee, Ja-Young | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.date.accessioned | 2021-01-31T09:19:22Z | - |
dc.date.available | 2021-01-31T09:19:22Z | - |
dc.date.created | 2020-04-06 | - |
dc.date.created | 2020-04-06 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | Archives of Biochemistry and Biophysics, Vol.679, p. 108162 | - |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.other | 94848 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172570 | - |
dc.description.abstract | Prostaglandin E-2 (PGE(2)) plays a key role in inflammation-associated carcinogenesis. NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) catalyzes the oxidation of the 15(S)-hydroxyl group of PGE(2) to generate 15-keto PGE(2). 15-PGDH has been known as a tumor suppressor in various malignancies including colon cancer. However, the molecular mechanisms underlying the tumor-suppressive function of 15-PGDH remain largely unresolved. In this study, we found that 15-keto PGE(2) upregulated the expression of heme oxygenase-1 (HO-1), a representative antioxidative and anti-inflammatory enzyme, at both transcriptional and translational levels, in human colon epithelial CCD 841 CoN cells. A redox-sensitive transcription factor, NF-E2-related factor (Nrf2) plays a critical role in the regulation of HO-1 and other cytoprotective proteins. 15-Keto PGE(2) induced translocation of Nrf2 into the nucleus and antioxidant response element-driven luciferase activity. Furthermore, the silencing of the Nrf2 gene abolished 15-keto PGE(2)-induced HO-1 expression in CCD 841 CoN cells. 15-Keto PGE(2) activated AKT signaling, and the pharmacological AKT inhibitor, LY294002 suppressed the 15-keto PGE(2)-induced HO-1 expression. 15-Keto PGE(2) generates the reactive oxygen species which is suppressed by the general antioxidant N-acetyl-L-cysteine. N-acetyl-L-cysteine treatment attenuated the 15-keto PGE(2)-induced phosphorylation of GSK3 beta, transcriptional activity of Nrf2, and subsequently HO-1 expression. However, 13,14-dihydro-15-keto PGE(2) lacking the alpha,beta-unsaturated carbonyl moiety failed to induce intracellular production of reactive oxygen species, HO-1 expression and nuclear translocation of Nrf2. In conclusion, 15-keto PGE(2) induces HO-1 expression through Nrf2 activation in human colon epithelial cells. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | 15-Keto prostaglandin E2 induces heme oxygenase-1 expression through activation of Nrf2 in human colon epithelial CCD 841 CoN cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1016/j.abb.2019.108162 | - |
dc.citation.journaltitle | Archives of Biochemistry and Biophysics | - |
dc.identifier.wosid | 000525443900002 | - |
dc.identifier.scopusid | 2-s2.0-85076837756 | - |
dc.citation.startpage | 108162 | - |
dc.citation.volume | 679 | - |
dc.identifier.sci | 000525443900002 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE | - |
dc.subject.keywordPlus | INCREASED SUSCEPTIBILITY | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | OXIDANT STRESS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | KEAP1 | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | PROGNOSIS | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | 15-Keto PGE(2) | - |
dc.subject.keywordAuthor | Nrf2 | - |
dc.subject.keywordAuthor | Heme oxygenase-1 | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Colon | - |
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