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Peroxynitrite induces HO-1 expression via PI3K/Akt-dependent activation of NF-E2-related factor 2 in PC12 cells
DC Field | Value | Language |
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dc.contributor.author | Li, Mei-Hua | - |
dc.contributor.author | Cha, Young-Nam | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-01-31T09:29:03Z | - |
dc.date.available | 2021-01-31T09:29:03Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2006-10 | - |
dc.identifier.citation | Free Radical Biology and Medicine, Vol.41 No.7, pp.1079-1091 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.other | 3644 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172718 | - |
dc.description.abstract | Peroxynitrite is a strong oxidant produced by rapid interaction between superoxide anion and nitric oxide radicals and induces oxidative stress and cell death. Treatment of PC12 cells with 3-morpholinosydnonimine (SIN-1), a generator of peroxynitrite, induced the expression of heme oxygenase-1 (HO-1), an antioxidant cytoprotective enzyme. Inhibition of the HO activity by zinc protoporphyrin IX or knockdown of HO-1 gene expression with siRNA exacerbated the SIN-1-induced apoptosis. After SIN-1 treatment, there was a time-related increase in nuclear localization and subsequent binding of NF-E2-related factor 2 (Nrf2) to the antioxidant-responsive element (ARE). Transfection of PC 12 cells with dominant-negative Nrf2 abolished the SIN-1-induced increase in Nrf2-ARE binding and subsequent upregulation of HO-1 expression, leading to enhanced cell death. Upon exposure of PC12 cells to SIN-1, the phosphatidylinositol 3-kinase (PI3K) activity was increased in a time-dependent manner. Pretreatment of cells with LY294002, a pharmacologic inhibitor of PI3K or transfection with the kinase-dead mutant Akt abrogated the SIN-1-induced Nrf2 activation and HO-1 expression. Taken together, these results suggest that peroxynitrite activates Nrf2 via PI3K/Akt signaling and enhances Nrf2-ARE binding, which leads to upregulation of HO-1 expression. The SIN-1-induced HO-1 upregulation may confer the adaptive survival response against nitrosative stress. (c) 2006 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Peroxynitrite induces HO-1 expression via PI3K/Akt-dependent activation of NF-E2-related factor 2 in PC12 cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2006.06.010 | - |
dc.citation.journaltitle | Free Radical Biology and Medicine | - |
dc.identifier.wosid | 000241142100008 | - |
dc.identifier.scopusid | 2-s2.0-33748310792 | - |
dc.citation.endpage | 1091 | - |
dc.citation.number | 7 | - |
dc.citation.startpage | 1079 | - |
dc.citation.volume | 41 | - |
dc.identifier.sci | 000241142100008 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 GENE | - |
dc.subject.keywordPlus | HUMAN NEUROBLASTOMA-CELLS | - |
dc.subject.keywordPlus | SMOOTH-MUSCLE-CELLS | - |
dc.subject.keywordPlus | ANTIOXIDANT-RESPONSIVE ELEMENT | - |
dc.subject.keywordPlus | NRF2 TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | CARBON-MONOXIDE | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordAuthor | peroxynitrite | - |
dc.subject.keywordAuthor | Heme oxygenase | - |
dc.subject.keywordAuthor | NF-E2-related factor 2 | - |
dc.subject.keywordAuthor | antioxidant responsive element | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | PC12 cells | - |
dc.subject.keywordAuthor | free radicals | - |
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