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cis-9,trans-11-Conjugated linoleic acid down-regulates phorbol ester-induced NF-κB activation and subsequent COX-2 expression in hairless mouse skin by targeting IκB kinase and PI3K-Akt : cis-9,trans-11-Conjugated linoleic acid down-regulates phorbol ester-induced NF-kappa B activation and subsequent COX-2 expression in hairless mouse skin by targeting I kappa B kinase and PI3K-Akt

DC Field Value Language
dc.contributor.authorHwang, Dal-Mi-
dc.contributor.authorKundu, Joydeb Kumar-
dc.contributor.authorShin, Jun-Wan-
dc.contributor.authorLee, Jung-Chul-
dc.contributor.authorLee, Hyong Joo-
dc.contributor.authorSurh, Young-Joon-
dc.date.accessioned2021-01-31T10:18:02Z-
dc.date.available2021-01-31T10:18:02Z-
dc.date.created2017-11-15-
dc.date.issued2007-02-
dc.identifier.citationCarcinogenesis, Vol.28 No.2, pp.363-371-
dc.identifier.issn0143-3334-
dc.identifier.other3529-
dc.identifier.urihttps://hdl.handle.net/10371/172801-
dc.description.abstractConjugated linoleic acid (CLA) has been reported to inhibit mouse skin carcinogenesis, particularly in the promotion stage, but underlying molecular mechanisms remain poorly understood. Since persistent induction of cyclooxygenase-2 (COX-2) is frequently implicated in carcinogenesis, we investigated the effect of cis-9,trans-11-CLA (9Z,11E-CLA) on the tumor promoter-induced COX-2 expression in HR-1 hairless mouse skin in vivo. Topical application of 9Z,11E-CLA caused significant inhibition of COX-2 expression at 6 h induced by 10 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) in HR-1 mouse skin. Since NF-kappa B is known to regulate COX-2 gene expression, we determined the effect of 9Z,11E-CLA on TPA-induced activation of this transcription factor. Treatment of mouse skin with 9Z,11E-CLA reduced TPA-induced DNA binding as well as nuclear translocation of NF-kappa B by blocking phosphorylation and subsequent degradation of I kappa B alpha. In addition, 9Z,11E-CLA attenuated TPA-induced phosphorylation of extracellular signal-regulated protein kinase, p38 mitogen-activated protein kinase and Akt. To further elucidate the molecular mechanism underlying the inactivation of NF-kappa B by 9Z,11E-CLA, we investigated its effect on TPA-induced activation of I kappa B kinase (IKK), an upstream kinase that regulates NF-kappa B via phosphorylation and degradation of I kappa B alpha. 9Z,11E-CLA treatment down-regulated phosphorylation and catalytic activities of IKK alpha/beta in TPA-treated mouse skin. Co-treatment of mouse skin with the IKK beta-specific inhibitor SC-514 (1 mu mol) attenuated TPA-induced activation of Akt and NF-kappa B, and also the expression of COX-2 in hairless mouse skin. Taken together, 9Z,11E-CLA inhibits NF-kappa B driven-COX-2 expression by blocking the IKK and PI3K-Akt signaling in TPA-treated hairless mouse skin in vivo, which may account for its previously reported anti-tumor promoting effects.-
dc.language영어-
dc.publisherOxford University Press-
dc.titlecis-9,trans-11-Conjugated linoleic acid down-regulates phorbol ester-induced NF-κB activation and subsequent COX-2 expression in hairless mouse skin by targeting IκB kinase and PI3K-Akt-
dc.title.alternativecis-9,trans-11-Conjugated linoleic acid down-regulates phorbol ester-induced NF-kappa B activation and subsequent COX-2 expression in hairless mouse skin by targeting I kappa B kinase and PI3K-Akt-
dc.typeArticle-
dc.contributor.AlternativeAuthor서영준-
dc.identifier.doi10.1093/carcin/bgl151-
dc.citation.journaltitleCarcinogenesis-
dc.identifier.wosid000244124800015-
dc.identifier.scopusid2-s2.0-33847277462-
dc.citation.endpage371-
dc.citation.number2-
dc.citation.startpage363-
dc.citation.volume28-
dc.identifier.sci000244124800015-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorLee, Hyong Joo-
dc.contributor.affiliatedAuthorSurh, Young-Joon-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusINFLAMMATION-ASSOCIATED CANCER-
dc.subject.keywordPlusSIGNAL-TRANSDUCTION PATHWAYS-
dc.subject.keywordPlusMAMMARY EPITHELIAL-CELLS-
dc.subject.keywordPlusANTIINFLAMMATORY PHYTOCHEMICALS-
dc.subject.keywordPlusTRANSACTIVATION DOMAIN-
dc.subject.keywordPlusMOLECULAR-MECHANISMS-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusP65 SUBUNIT-
dc.subject.keywordPlusCYCLOOXYGENASE-2-
dc.subject.keywordPlusPHOSPHORYLATION-
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