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Puerarin suppresses AGEs-induced inflammation in mouse mesangial cells: A possible pathway through the induction of heme oxygenase-1 expression
DC Field | Value | Language |
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dc.contributor.author | Kim, Ki Mo | - |
dc.contributor.author | Jung, Dong Ho | - |
dc.contributor.author | Jang, Dae Sik | - |
dc.contributor.author | Kim, Young Sook | - |
dc.contributor.author | Kim, Jong Min | - |
dc.contributor.author | Kim, Ha-Na | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.contributor.author | Kim, Jin Sook | - |
dc.date.accessioned | 2021-01-31T10:25:02Z | - |
dc.date.available | 2021-01-31T10:25:02Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | Toxicology and Applied Pharmacology, Vol.244 No.2, pp.106-113 | - |
dc.identifier.issn | 0041-008X | - |
dc.identifier.other | 2624 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172913 | - |
dc.description.abstract | Puerarin is a natural product isolated from Puerarin lobata and has various pharmacological effects, including anti-hyperglycemic and anti-allergic properties. In the present study, we investigated the effect of puerarin against advanced glycation end products (AGEs)-induced inflammation in mouse mesangial cells. Puerarin acts by inducing the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Puerarin was able to enhance phosphorylation of protein kinase C (PKC) delta, but not PKC alpha/beta II, in a time-dependent manner. Induction of HO-1 expression by puerarin was suppressed by GF109203X, a general inhibitor of PKC, and by rottlerin, a specific inhibitor of PKC delta. However, induction was not suppressed by Go6976, a selective inhibitor for PKC alpha/beta II. Additionally, the knockdown of endogenous PKC delta by small interfering RNA (siRNA) resulted in the inhibition of HO-1 expression and Akt phosphorylation. Puerarin increased antioxidant response element (ARE)-Luciferase activity in a dose- and time-dependent manner in transfected mouse mesangial cells. Mutation of the ARE sequence abolished puerarin-induced HO-1 expression. Furthermore, puerarin treatments resulted in a marked increase in NF-E2 related factor-2 (Nrf-2) translocation, leading to up-regulation of HO-1 expression. However, transfection of Nrf-2 specific siRNA abolished HO-1 expression. Pretreatment with puerarin inhibited the expressions of COX-2, MMP-2 and MMP-9. But, these effects were reversed by ZnPP, an inhibitor of HO-1. Taken together, our results demonstrate that puerarin-induced expression of HO-1 is mediated by the PKC delta-Nrf-2-HO-1 pathway and inhibits N-carboxymethyllysine (CML)-induced inflammation in mouse mesangial cells. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Puerarin suppresses AGEs-induced inflammation in mouse mesangial cells: A possible pathway through the induction of heme oxygenase-1 expression | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1016/j.taap.2009.12.023 | - |
dc.citation.journaltitle | Toxicology and Applied Pharmacology | - |
dc.identifier.wosid | 000276664800002 | - |
dc.identifier.scopusid | 2-s2.0-77950033149 | - |
dc.citation.endpage | 113 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 106 | - |
dc.citation.volume | 244 | - |
dc.identifier.sci | 000276664800002 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GLYCATION END-PRODUCTS | - |
dc.subject.keywordPlus | HUMAN NEUROBLASTOMA-CELLS | - |
dc.subject.keywordPlus | INDUCED DIABETIC-RATS | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | RESPONSIVE ELEMENTS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | OXIDANT STRESS | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordAuthor | Puerarin | - |
dc.subject.keywordAuthor | Heme oxygenase-1 | - |
dc.subject.keywordAuthor | Nuclear E2-factor related factor 2 | - |
dc.subject.keywordAuthor | N-carboxymethyllysine | - |
dc.subject.keywordAuthor | Inflammation | - |
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