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Adenovirus-uteroglobin suppresses COX-2 expression via inhibition of NF-κB activity in lung cancer cells

Cited 15 time in Web of Science Cited 15 time in Scopus
Authors
Yoon, Jung Min; Lim, Jae-Jun; Yoo, Chul-Gyu; Lee, Choon-Taek; Bang, Yung-Jue; Han, Sung Koo; Shim, Young-Soo; Kim, Young Whan
Issue Date
2005-05
Citation
Lung Cancer, Vol.48 No.2, pp.201-209
Keywords
uterogtobinlung cancerCOX-2NF-kappa Badenovirusgene therapy
Abstract
Uteroglobin (UG, Clara cell secretory protein) is a steroid inducible, multifunctional protein that is secreted by the mucosal epithelia. UG has anti-proliferative and anti-metastatic effects in cancer cells. COX-2, which catalyzes the first step in the synthesis of prostanoids, has been shown to be overexpressed in tumors. This study investigated the effect of UG on the inhibition of COX-2 expression in lung cancer cells. The level of the COX-2 protein and its mRNA were decreased by UG, as demonstrated by Western Not and the RT-PCR, respectively. The EIA shows that UG suppressed PGE2 synthesis. Western blot showed that the NF-kappa B nuclear translocation was inhibited by the transduction of UG. In addition, an EMSA demonstrated the inhibition of the NF-kappa B-DNA binding by UG. The Luciferase assay showed that UG also inhibited the NF-kappa B-mediated transcription activity. Furthermore, transfection of the lung cancer cell lines with the COX-2 reporter gene constructs demonstrated that the transcription of COX-2 gene was suppressed by UG. These results show that the inhibition of COX-2 expression by UG transduction correlated with the suppression of NF-kappa B activity in the Lung cancer cells. This suggests that UG have the possibility for the treatment of lung cancer. (c) 2004 Published by Elsevier Ireland Ltd.
ISSN
0169-5002
URI
https://hdl.handle.net/10371/173026
DOI
https://doi.org/10.1016/j.lungcan.2004.11.005
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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