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Cdk2-dependent phosphorylation of the NF-Y transcription factor is essential for the expression of the cell cycle-regulatory genes and cell cycle G1/S and G2/M transitions
DC Field | Value | Language |
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dc.contributor.author | Chae, Hee-Don | - |
dc.contributor.author | Yun, Jaenho | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.contributor.author | Shin, Deug Y. | - |
dc.date.accessioned | 2021-01-31T12:00:19Z | - |
dc.date.available | 2021-01-31T12:00:19Z | - |
dc.date.created | 2020-12-18 | - |
dc.date.issued | 2004-05 | - |
dc.identifier.citation | Oncogene, Vol.23 No.23, pp.4084-4088 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.other | 119275 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173200 | - |
dc.description.abstract | We previously reported that cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y transcription factor and this phosphorylation is essential for DNA binding of NF-Y. In this study, we examined the effects of a phosphorylation-deficient mutant form of YA, YA-aa, in which the two serine residues are replaced with alanine, on the cell cycle and expression of the NF-Y target genes. Transient transfection assays show that YA-aa inhibits transcription from the NF-Y target promoters, such as cdc2, cyclin A, and cdc25C. Moreover, this inhibitory function of YA-aa can be suppressed by the expression of wild-type YA, implying that YA-aa inhibits transcription of those NF-Y target genes by inactivating wild-type YA. Since NF-Y target genes include the cell cycle-regulatory genes that ensure orderly progression of the cell cycle, we examined the effects of YA-aa in cell cycle progression. We constructed a recombinant adenovirus encoding YA-aa and found that YA-aa expression leads to repression of cell cycle-regulatory genes, such as cyclin A, RNR R2, DNA polymerase a, cdc2, cyclin B, and cdc25C. Consistently, YA-aa expression results in the inactivation of both cdc2 and cdk2. Furthermore, cell cycle analysis reveals that YA-aa induces cell cycle arrest at both G1 and G2/M. These results suggest that cdk2-dependent phosphorylation of NF-Y is essential for the expression of the cell cycle-regulatory genes and therefore for cell cycle progression at both G1/S and G2/M. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Cdk2-dependent phosphorylation of the NF-Y transcription factor is essential for the expression of the cell cycle-regulatory genes and cell cycle G1/S and G2/M transitions | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1038/sj.onc.1207482 | - |
dc.citation.journaltitle | Oncogene | - |
dc.identifier.wosid | 000221520200006 | - |
dc.identifier.scopusid | 2-s2.0-2942614689 | - |
dc.citation.endpage | 4088 | - |
dc.citation.number | 23 | - |
dc.citation.startpage | 4084 | - |
dc.citation.volume | 23 | - |
dc.identifier.sci | 000221520200006 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CCAAT-BINDING PROTEIN | - |
dc.subject.keywordPlus | MACROPHAGE DIFFERENTIATION | - |
dc.subject.keywordPlus | G(2) ARREST | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SENESCENCE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | NF-Y | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | cdc2 | - |
dc.subject.keywordAuthor | cdk2 | - |
dc.subject.keywordAuthor | G1/G2 | - |
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