Browse

Analysis of mutations of neurofibromatosis type 1 gene and N‐ras gene in acute myelogenous leukemia

Cited 17 time in Web of Science Cited 21 time in Scopus
Authors
Lee, Young Y; Kim, Won S.; Bang, Yung‐J.; Jung, Chul W.; Park, Seonyang; Yoon, Whi J.; Cho, Kyoung S.; Kim, In S.; Jung, Tae J.; Choi, Il Y.; Kim, Byoung K.; Kim, Noe K.; Koeffler, H. Phillip
Issue Date
1995-09
Citation
Stem Cells, Vol.13 No.5, pp.556-563
Keywords
MUTATIONFLR EXONNF1GTPASE-ACTIVATING PROTEINNEUROFIBROMINN-RASACUTE MYELOGENOUS LEUKEMIA
Abstract
Neurofibromatosis type 1 (NF1) gene is a tumor suppressor gene, and the NF1 gene product, neurofibromin, can downregulate the N-ras gene. Because the N-ras gene is often mutated in acute myelogenous leukemia (AML), we wondered if the NF1 gene might be mutated in those AML samples not having N-ras mutations. We investigated the mutational status of the N-ras gene and the FLR exon of codons 1371-1423 of the open reading frame of the full-length NF1 cDNA, which has a strong homology with the mammalian ras GTPase-activating protein (GAP), especially for a stretch of three consecutive amino acids (F, L, R), by single-strand conformation polymorphism analysis and direct sequencing in samples from patients with AML. Of 48 AML patients, 10 (21%) had point (missense) mutations of the N-ras gene involving codons 12, 13 and 61. However, mutations in the FLR exon of the NF1 gene were not detected in any of the AML samples. We also examined the difference of clinical response to induction therapy between AML patients with and without N-ras mutation. A significantly lower rate of complete remission was noted in individuals with the N-ras gene mutations. These results suggest that mutation of the NF1 gene, at least in the FLR exon, is very rare in AML and the NF1 gene probably is not a functional complement of the N-ras gene mutation. The presence of N-ras gene mutation may be associated with a lower clinical response to antileukemic therapy.
ISSN
1066-5099
URI
https://hdl.handle.net/10371/173255
DOI
https://doi.org/10.1002/stem.5530130514
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse