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Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Gang | - |
dc.contributor.author | He, Dan | - |
dc.contributor.author | Zhao, Fengchun | - |
dc.contributor.author | Hu, Junqiang | - |
dc.contributor.author | Lee, Yin-Won | - |
dc.contributor.author | Shi, Jianrong | - |
dc.contributor.author | Xu, Jianhong | - |
dc.date.accessioned | 2021-03-23T04:56:39Z | - |
dc.date.available | 2021-03-23T13:57:43Z | - |
dc.date.issued | 2020-12-08 | - |
dc.identifier.citation | Food Production, Processing and Nutrition. 2020 Dec 08;2(1):29 | ko_KR |
dc.identifier.issn | 2661-8974 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173893 | - |
dc.description.abstract | Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. | ko_KR |
dc.description.sponsorship | This work was financially supported by the National Key Research and Development Project (2018YFE0206000), National Natural Science Foundation of China (31901805, U1604234, 31872914) and Jiangsu Agriculture Science and Technology Innovation Fund (CX (19)3004). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Ustiloxin A | - |
dc.subject | Rice false smut | - |
dc.subject | Preparative purification | - |
dc.subject | Macroporous resin | - |
dc.subject | High-speed counter-current chromatography | - |
dc.subject | Phytotoxicity assay | - |
dc.title | Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | 10.1186/s43014-020-00043-9 | - |
dc.citation.journaltitle | Food Production, Processing and Nutrition | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2021-01-27T10:46:32Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 29 | ko_KR |
dc.citation.volume | 2 | ko_KR |
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