Browse

Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Jeong, Yeong Yeop.; Lee, Hun-Young; Kim, Suk Weon; Noh, Yoo-Sun; Seo, Pil Joon
Issue Date
2021-02-23
Publisher
BMC
Citation
Plant Methods. 2021 Feb 23;17(1):21
Keywords
ProtoplastPlant regenerationPluripotencyCell divisionDe novo organogenesis
Abstract
Background
Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration. With the growing need for protoplast regeneration in genetic engineering and genome editing, fundamental studies that enhance our understanding of cell cycle re-entry, pluripotency acquisition, and de novo tissue regeneration are essential. To conduct these studies, a reproducible and efficient protoplast regeneration method using model plants is necessary.

Results
Here, we optimized cell and tissue culture methods for improving protoplast regeneration efficiency in Arabidopsis thaliana. Protoplasts were isolated from whole seedlings of four different Arabidopsis ecotypes including Columbia (Col-0), Wassilewskija (Ws-2), Nossen (No-0), and HR (HR-10). Among these ecotypes, Ws-2 showed the highest potential for protoplast regeneration. A modified thin alginate layer was applied to the protoplast culture at an optimal density of 1 × 106 protoplasts/mL. Following callus formation and de novo shoot regeneration, the regenerated inflorescence stems were used for de novo root organogenesis. The entire protoplast regeneration process was completed within 15 weeks. The in vitro regenerated plants were fertile and produced morphologically normal progenies.

Conclusion
The cell and tissue culture system optimized in this study for protoplast regeneration is efficient and reproducible. This method of Arabidopsis protoplast regeneration can be used for fundamental studies on pluripotency establishment and de novo tissue regeneration.
ISSN
1746-4811
Language
English
URI
https://hdl.handle.net/10371/174356
DOI
doi.org/10.1186/s13007-021-00720-x
Files in This Item:
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse