S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dental Science(치의과학과) Journal Papers (저널논문_치의과학과)
DARPP-32의 인간 신경줄기세포 증식과 생존에서의 역할 규명
Role of DARPP-32 in the Proliferation and Survival of Human Neural Stem Cells
- Issue Date
- 대한구강해부학회지, Vol.41 No.1, pp.97-108
- To investigate the role of dopamine- and cAMP-regulated neuronal phosphoprotein 32 kDa (DARPP-32), also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), in cell proliferation and survival of human neural stem cells (hiNSCs) induced from human adipose-derived stem cells, DARPP-32 expression was knocked down by a lentiviral vector. Cell proliferation was relatively slower and doubling time was longer in the DARPP-32-knockdown hNSCs than in the scrambled control. Oxidative stress testing showed that DARPP-32 knockdown caused more cell death in hiNSCs after hydrogen peroxide (H2O2) treatment than in the scrambled control. The expressions of CTNNB1, CXCR4, and VCAM-1, which are related to cell proliferation, were lower in the DARPP-32-knockdown hiNSCs than in the scrambled control. In addition, DARPP-32 knockdown decreased the expression of Bcl-2 and increased the expression of Bax in response to H2O2 treatment.
We also confirmed reduced expression of phosphorylated Akt (p-AKT) in DARPP-32-knockdown hiNSCs.
In conclusion, these findings demonstrate the mechanisms by which DARPP-32 positively affects cell proliferation and survival in hiNSCs.
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