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Whole-genome sequencing and analysis of Streptomyces strains producing multiple antinematode drugs
DC Field | Value | Language |
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dc.contributor.author | Yi, Jeong Sang | - |
dc.contributor.author | Kim, Jung Min | - |
dc.contributor.author | Kang, Min-Kyoung | - |
dc.contributor.author | Kim, Jong Hoon | - |
dc.contributor.author | Cho, Hang Su | - |
dc.contributor.author | Ban, Yeon Hee | - |
dc.contributor.author | Song, Myoung Chong | - |
dc.contributor.author | Son, Kwang-Hee | - |
dc.contributor.author | Yoon, Yeo Joon | - |
dc.date.accessioned | 2022-09-19T06:27:05Z | - |
dc.date.available | 2022-09-19T15:28:49Z | - |
dc.date.issued | 2022-08-23 | - |
dc.identifier.citation | BMC Genomics, 23(1):610 | ko_KR |
dc.identifier.issn | 1471-2164 | - |
dc.identifier.uri | https://doi.org/10.1186/s12864-022-08847-4 | - |
dc.identifier.uri | https://hdl.handle.net/10371/184489 | - |
dc.description.abstract | Background : Nematodes are parasitic animals that cause over 100 billion US dollars loss in agricultural business. The whole-genomes of two Streptomyces strains, Streptomyces spectabilis KCTC9218T and Streptomyces sp. AN091965, were sequenced. Both strains produce spectinabilin, an antinematode drug. Its secondary metabolism was examined to aid the development of an efficient nematicidal drug-producing host strain.
Results : The whole-genome sequences of S. spectabilis KCTC9218T and Streptomyces sp. AN091965 were analyzed using PacBio and Illumina sequencing platforms, and assembled using hybrid methodology. The total contig lengths for KCTC9218T and AN091965 were 9.97Mb and 9.84Mb, respectively. A total of 8,374 and 8,054 protein-coding genes, as well as 39 and 45 secondary metabolite biosynthetic gene clusters were identified in KCTC9218T and AN091965, respectively. 18.4 ± 6.45mg/L and 213.89 ± 21.30mg/L of spectinabilin were produced by S. spectabilis KCTC9218T and Streptomyces sp. AN091965, respectively. Pine wilt disease caused by nematode was successfully prevented by lower concentration of spectinabilin injection than that of abamectin recommended by its manufacturer. Production of multiple antinematode drugs, including spectinabilin, streptorubin B, and undecylprodigiosin was observed in both strains using high-resolution liquid chromatography mass spectrometry (LC–MS) analysis. Conclusions : Whole-genome sequencing of spectinabilin-producing strains, coupled with bioinformatics and mass spectrometry analyses, revealed the production of multiple nematicidal drugs in the KCTC9218T and AN091965 strains. Especially, Streptomyces sp. AN091965 showed high production level of spectinabilin, and this study provides crucial information for the development of potential nematicidal drug producers. | ko_KR |
dc.description.sponsorship | This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) throughCrop Viruses and Pests Response Industry Technology Development Program (No.321110–4) funded by Ministry of Agriculture, Food and Rural Afairs (MAFRA), National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No.2022R1A2C3004621) and the Ministry of Education (2022R1I1A1A01068507), and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2020M3H1A1073304). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Streptomyces | - |
dc.subject | Secondary metabolite | - |
dc.subject | Nematicidal | - |
dc.subject | Spectinabilin | - |
dc.subject | Undecylprodigiosin | - |
dc.title | Whole-genome sequencing and analysis of Streptomyces strains producing multiple antinematode drugs | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | 10.1186/s12864-022-08847-4 | ko_KR |
dc.citation.journaltitle | BMC Genomics | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-08-28T03:12:30Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 610 | ko_KR |
dc.citation.volume | 23 | ko_KR |
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