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The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury

Cited 305 time in Web of Science Cited 332 time in Scopus
Authors

Oh, Joo Youn; Kim, Mee Kum; Shin, Mi Sun; Lee, Hyun Ju; Ko, Jung Hwa; Wee, Won Ryang; Lee, Jin Hak

Issue Date
2008-04
Publisher
AlphaMed Press Inc
Citation
Stem Cells, Vol.26 No.4, pp.1047-1055
Abstract
To investigate the anti-inflammatory and anti-angiogenic effects of mesenchymal stem cells (MSC) in the chemically burned corneas, we mechanically removed the corneal epithelium of rats after 100% alcohol instillation. The rats were then randomized into four groups: fresh media, conditioned media derived from the MSC culture (MSC-CM), MSC applied topically to the damaged corneas for 2 hours immediately after the injury or MSC-CM applied either once or 3 times per day for 3 consecutive days. Corneal surface was evaluated every week. After 3 weeks, the corneas were stained with the hematoxylineosin, and the expression of interleukin (IL)-2, interferon (IFN)-gamma, EL-6, IL-10, transforming growth factor (TGF)-beta 1, thrombospondin-1 (TSP-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor (VEGF) were analyzed. CD4(+) cells were assessed in the corneas. We found that both MSC and three-time applied MSC-CM (1) reduced corneal inflammation and neovascularization, (2) decreased IIL-2 and IEFN-gamma, although increased IIL-10 and TGF-beta 1. as well as IL-6, (3) reduced the infiltration of CD4(+) cells, and (4) upregulated the expression of TSP-1, although downregulated that of MMP-2. Interestingly, whereas three-time application of MSC-CM was partially effective, transplantation of MSC achieved a better outcome in suppressing corneal inflammation. The results of this study suggest that the anti-inflammatory and anti-angiogenic action of MSC in the chemically burned corneas might be mediated in part through paracrine pathways involving soluble factors such as IL-10, TGF-beta 1, IL-6 and TSP-1.
ISSN
1066-5099
URI
https://hdl.handle.net/10371/191690
DOI
https://doi.org/10.1634/stemcells.2007-0737
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  • College of Medicine
  • Department of Medicine
Research Area 각막 및 외안부 질환, 백내장

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