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OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools

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Authors

Lee, Ara; Sung, Gihyun; Shin, Sanghee; Lee, Song-Yi; Sim, Jaehwan; Nhung, Truong Thi My; Nghi, Tran Diem; Park, Sang Ki; Sathieshkumar, Ponnusamy Pon; Kang, Imkyeung; Mun, Ji Young; Kim, Jong-Seo; Rhee, Hyun-Woo; Park, Kyeng Min; Kim, Kimoon

Issue Date
2024-02
Publisher
Nature Publishing Group
Citation
Nature Communications, Vol.15 No.1, p. 1851
Abstract
Identifying proteins at organelle contact sites, such as mitochondria-associated endoplasmic reticulum membranes (MAM), is essential for understanding vital cellular processes, yet challenging due to their dynamic nature. Here we report OrthoID, a proteomic method utilizing engineered enzymes, TurboID and APEX2, for the biotinylation (Bt) and adamantylation (Ad) of proteins close to the mitochondria and endoplasmic reticulum (ER), respectively, in conjunction with high-affinity binding pairs, streptavidin-biotin (SA-Bt) and cucurbit[7]uril-adamantane (CB[7]-Ad), for selective orthogonal enrichment of Bt- and Ad-labeled proteins. This approach effectively identifies protein candidates associated with the ER-mitochondria contact, including LRC59, whose roles at the contact site were—to the best of our knowledge—previously unknown, and tracks multiple protein sets undergoing structural and locational changes at MAM during mitophagy. These findings demonstrate that OrthoID could be a powerful proteomics tool for the identification and analysis of spatiotemporal proteins at organelle contact sites and revealing their dynamic behaviors in vital cellular processes.
ISSN
2041-1723
URI
https://hdl.handle.net/10371/201872
DOI
https://doi.org/10.1038/s41467-024-46034-z
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  • College of Natural Sciences
  • School of Biological Sciences
Research Area Molecular Interactomics, Proteomics, Systems Biology, 단백체학, 분자상호작용체학, 시스템생물학

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