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Chemokine receptor 5 blockade modulates macrophage trafficking in renal ischaemic-reperfusion injury

Cited 8 time in Web of Science Cited 10 time in Scopus
Authors

Yoo, Kyung Don; Cha, Ran-hui; Lee, Sunhwa; Kim, Ji Eun; Kim, Kyu Hong; Lee, Jong Soo; Kim, Dong Ki; Kim, Yon Su; Yang, Seung Hee

Issue Date
2020-05
Publisher
Blackwell Publishing Inc.
Citation
Journal of Cellular and Molecular Medicine, Vol.24 No.10, pp.5515-5527
Abstract
Chemokine receptor 5 (CCR5) is a pivotal regulator of macrophage trafficking in the kidneys in response to an inflammatory cascade. We investigated the role of CCR5 in experimental ischaemic-reperfusion injury (IRI) pathogenesis. To establish IRI, we clamped the bilateral renal artery pedicle for 30 min and then reperfused the kidney. We performed adoptive transfer of lipopolysaccharide (LPS)-treated RAW 264.7 macrophages following macrophage depletion in mice. B6.CCR5(-/-) mice showed less severe IRI based on tubular epithelial cell apoptosis than did wild-type mice. CXCR3 expression in CD11b(+) cells and inducible nitric oxide synthase levels were more attenuated in B6.CCR5(-/-) mice. B6.CCR5(-/-) mice showed increased arginase-1 and CD206 expression. Macrophage-depleted wild-type mice showed more injury than B6.CCR5(-/-) mice after M1 macrophage transfer. Adoptive transfer of LPS-treated RAW 264.7 macrophages reversed the protection against IRI in wild-type, but not B6.CCR5(-/-) mice. Upon knocking out CCR5 in macrophages, migration of bone marrow-derived macrophages from wild-type mice towards primary tubular epithelial cells with recombinant CCR5 increased. Phospho-CCR5 expression in renal tissues of patients with acute tubular necrosis was increased, showing a positive correlation with tubular inflammation. In conclusion, CCR5 deficiency favours M2 macrophage activation, and blocking CCR5 might aid in treating acute kidney injury.
ISSN
1582-1838
URI
https://hdl.handle.net/10371/205990
DOI
https://doi.org/10.1111/jcmm.15207
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  • College of Medicine
  • Department of Medicine
Research Area Nephrology, Transplantation, Urology

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