Usefulness of Three-Channel Multiplex Real-Time PCR and Melting Curve Analysis for Simultaneous Detection and Identification of the Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria

Hong, Yun Ji; Chung, Young Hoon; Kim, Taek Soo; Song, Sang Hoon; Park, Kyoung Un; Yim, Jae Joon; Song, Junghan; Lee, Jae Ho; Kim, Eui Chong

doi:10.1128/JCM.05662-11

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Usefulness of Three-Channel Multiplex Real-Time PCR and Melting Curve Analysis for Simultaneous Detection and Identification of the Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria

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Authors: Hong, Yun Ji; Chung, Young Hoon; Kim, Taek Soo; Song, Sang Hoon; Park, Kyoung Un; Yim, Jae Joon; Song, Junghan; Lee, Jae Ho; Kim, Eui Chong

Issue Date: 2011-11

Publisher: American Society for Microbiology

Citation: Journal of Clinical Microbiology, Vol.49 No.11, pp.3963-3966

Abstract: We attempted to determine the benefits of three-channel multiplex real-time PCR and melting curve analysis not only in detecting and distinguishing between nontuberculous mycobacteria (NTM) and the Mycobacterium tuberculosis complex but also in identifying NTM to the species level.