MC3 점액표피양 암종세포에서 비타민 K2의 항암효과

Abstract

Background. Vitamin K (VK) is a fat-soluble vitamin and is known to have anticancer activity in various cancer cell lines. However,there is no report on the anticancer effect of VK2 in mucoepidermoid carcinoma cells. Methods. The effects of VK2 on anti-proliferativeand apoptotic activity were recognized by the trypan blue exclusion assay, 4'-6-diamidino-2-phenylindole (DAPI) staining and Westernblot analysis. Results. The results showed that VK2 decreased cell viability and induced apoptotic programmed cell death in MC3 cellsevidenced by the cleavages of caspase3 and PARP. VK2 treatment clearly increased Bak and truncated Bid (t-Bid) compared withthe control treatment whereas it did not alter other Bcl-2 family members. Conclusions. Overall, our results suggest that VK2 can bea good apoptotic inducer accompanied by the increase in Bak and Bid protein. VK2 may be a potent target of anticancer drug candidatefor the treatment of oral cancer.