BARODON 에 의한 Human Gastric Adenocarcinoma AGS 세포고사

Abstract

BARODON?is a multi-purpose, high functional alkali solution made by mixing and liquid- ionizing silicon, calcium, sodium, borax, organic carbon chemicals and silver. In this study, we have investigated the apoptotic potential and mechanistic insights of BARODON?in human gastric cancer cell line (AGS cells). In MTT assay, BARODON?reduced cell viability in AGS cells. Morphological features of apoptosis with marked cytoplasmic vacuolation and appearance of apoptotic peaks in flow cytometry were observed in AGS cells with BARODON?treatment. In addition, BARODON?induced apoptosis of stomach cancer cell is related to bax up-regulation, caspase 7 protease activation and subsequent cleavage of poly (ADP-ribose) polymerase (PARP). These results suggest that BARODON?can induce the apoptosis of AGS cells through modulation of bcl-2 family and the activation of intrinsic caspase cascades, indicating that it is potentially useful as a anti-cancer agent.