봉독 추출물(F1,F3)의 랫드에 대한 단회 및 4주 반복 피하 투여 독성시험

Abstract

This study was performed to evaluate single and repeated-dose toxicities of BeVenom Extracts (F1, F3) in Spraque-Dawley. F1 was injected subcutaneously to rat at dose levels of0, 0.0002, 0.002, 0.02 mg/kg/day for single-dose toxicity study and repeated-dose toxicity study. F3was injected subcutaneously to rat at dose level of 0, 0.003, 0.03, 0.3 mg/kg/day for single-dose toxic-ity study and repeated-dose toxicity study. In both studies, there were no dose related changes inmortality, clinical sign, body weight change, food and water consumption, opthalmoscopy, organweights, urine analysis, biochemical examination, and hematological findings of all animals treatedwith Bee Venom (F1, F3). Gross and histopathological findings revealed no evidence of specific toxic-ity related to Bee Venom (F1, F3). These results suggest that the subcutaneous NOEL (No ObservedEffect Level) of Bee Venom (F1, F3) may be over F1 - 0.02 mg/kg, F3 - 0.3 mg/kg.