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양전자 방출핵종 68Ga을 이용한 NOTA와 DOTA의 표지 및 시험관내 특성 연구 : Radiolabeling of NOTA and DOTA with Positron Emitting 68Ga and
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Jae Min | - |
dc.contributor.author | Kim, Young Ju | - |
dc.contributor.author | Lee, Yun-Sang | - |
dc.contributor.author | Lee, Dong Soo | - |
dc.contributor.author | Chung, June-Key | - |
dc.contributor.author | Lee, Myung Chul | - |
dc.date.accessioned | 2009-12-17T08:17:28Z | - |
dc.date.available | 2009-12-17T08:17:28Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Nucl Med Mol Imaging 2009;43(4):330-336 | en |
dc.identifier.issn | 1975-129X | - |
dc.identifier.uri | http://ksnm.or.kr/journal/view.php?year=2009&vol=43&no=4&startpage=330 | - |
dc.identifier.uri | https://hdl.handle.net/10371/21270 | - |
dc.description.abstract | Purpose: We established radiolabeling conditions of NOTA and DOTA with a generator-produced PET radionuclide
68Ga and studied in vitro characteristics such as stability, serum protein binding, octanol/water distribution, and interference with other metal ions. Materials and Methods: Various concentrations of NOTA․3HCl and DOTA․ 4HCl were labeled with 1 mL 68GaCl3 (0.18~5.75 mCi in 0.1 M HCl) in various pH. NOTA․3HCl (0.373 mM) was labeled with 68GaCl3 (0.183~0.232 mCi/0.1 M HCl 1.0 mL) in the presense of CuCl2, FeCl2, InCl3, FeCl3, GaCl3, MgCl2 or CaCl2 (0~6.07 mM) at room temperature. The labeling efficiencies of 68Ga-NOTA and 68Ga-DOTA were checked by ITLC-SG using acetone or saline as mobile phase. Stabilities, protein bindings, and octanol distribution coefficients of the labeled compounds also were investigated. Results: 68Ga-NOTA and 68Ga-DOTA were labeled optimally at pH 6.5 and pH 3.5, respectively, and the chelates were stable for 4 hr either in the reaction mixture at room temperature or in the human serum at 37°C. NOTA was labeled at room temperature while DOTA required heating for labeling. 68Ga-NOTA labeling efficiency was reduced by CuCl2, FeCl2, InCl2, FeCl3 or GaCl3, however, was not influenced by MgCl2 or CaCl2. The protein binding was low (2.04~3.32%). Log P value of 68Ga-NOTA was -3.07 indicating high hydrophilicity. Conclusion: We found that NOTA is a better bifunctional chelating agent than DOTA for 68Ga labeling. Although, 68Ga-NOTA labeling is interfered by various metal ions, it shows high stability and low serum protein binding. | en |
dc.description.sponsorship | 한국과학재단 국가지정연구실사업 (R0A-2008-000-
20116-0) 및 원자력연구개발사업 (2007-01238) | en |
dc.language.iso | ko | - |
dc.publisher | 대한핵의학회 = The Korean Society of Nuclear Medicine | en |
dc.subject | Ga-68 | en |
dc.subject | gallium | en |
dc.subject | protein binding | en |
dc.subject | bifunctional chelating agent | en |
dc.subject | generator | en |
dc.subject | NOTA | en |
dc.subject | DOTA | en |
dc.title | 양전자 방출핵종 68Ga을 이용한 NOTA와 DOTA의 표지 및 시험관내 특성 연구 | en |
dc.title.alternative | Radiolabeling of NOTA and DOTA with Positron Emitting 68Ga and | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정재민 | - |
dc.contributor.AlternativeAuthor | 김영주 | - |
dc.contributor.AlternativeAuthor | 이윤상 | - |
dc.contributor.AlternativeAuthor | 이동수 | - |
dc.contributor.AlternativeAuthor | 정준기 | - |
dc.contributor.AlternativeAuthor | 이명철 | - |
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