Publications

Detailed Information

Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts

DC Field Value Language
dc.contributor.authorKim, Sang Wan-
dc.contributor.authorHer, Sun Ju-
dc.contributor.authorKim, Seong Yeon-
dc.contributor.authorShin, Chan Soo-
dc.date.accessioned2009-12-23T05:54:52Z-
dc.date.available2009-12-23T05:54:52Z-
dc.date.issued2005-01-15-
dc.identifier.citationBiochem Biophys Res Commun. 2005 Feb 18;327(3):811-9.en
dc.identifier.issn0006-291X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15649418-
dc.identifier.urihttps://hdl.handle.net/10371/22149-
dc.description.abstractOsteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes.en
dc.language.isoenen
dc.publisherElsevieren
dc.subject1-Methyl-3-isobutylxanthine/pharmacologyen
dc.subject3T3 Cellsen
dc.subjectAdipocytes/*metabolismen
dc.subjectAlkaline Phosphatase/metabolismen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectCCAAT-Enhancer-Binding Protein-alpha/metabolismen
dc.subjectCell Differentiation/*physiologyen
dc.subjectCells, Cultureden
dc.subjectDexamethasone/pharmacologyen
dc.subjectGene Expression Regulationen
dc.subjectInsulin/metabolismen
dc.subjectMiceen
dc.subjectOsteoblasts/*cytologyen
dc.subjectPPAR gamma/metabolismen
dc.subjectPhenotypeen
dc.subjectTranscription Factors/genetics/*physiologyen
dc.titleEctopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblastsen
dc.typeArticleen
dc.contributor.AlternativeAuthor김상완-
dc.contributor.AlternativeAuthor허선주-
dc.contributor.AlternativeAuthor김성연-
dc.contributor.AlternativeAuthor심찬수-
dc.identifier.doi10.1016/j.bbrc.2004.12.076-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share