Publications
Detailed Information
Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sang Wan | - |
dc.contributor.author | Her, Sun Ju | - |
dc.contributor.author | Kim, Seong Yeon | - |
dc.contributor.author | Shin, Chan Soo | - |
dc.date.accessioned | 2009-12-23T05:54:52Z | - |
dc.date.available | 2009-12-23T05:54:52Z | - |
dc.date.issued | 2005-01-15 | - |
dc.identifier.citation | Biochem Biophys Res Commun. 2005 Feb 18;327(3):811-9. | en |
dc.identifier.issn | 0006-291X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15649418 | - |
dc.identifier.uri | https://hdl.handle.net/10371/22149 | - |
dc.description.abstract | Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | 1-Methyl-3-isobutylxanthine/pharmacology | en |
dc.subject | 3T3 Cells | en |
dc.subject | Adipocytes/*metabolism | en |
dc.subject | Alkaline Phosphatase/metabolism | en |
dc.subject | Animals | en |
dc.subject | Base Sequence | en |
dc.subject | CCAAT-Enhancer-Binding Protein-alpha/metabolism | en |
dc.subject | Cell Differentiation/*physiology | en |
dc.subject | Cells, Cultured | en |
dc.subject | Dexamethasone/pharmacology | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Insulin/metabolism | en |
dc.subject | Mice | en |
dc.subject | Osteoblasts/*cytology | en |
dc.subject | PPAR gamma/metabolism | en |
dc.subject | Phenotype | en |
dc.subject | Transcription Factors/genetics/*physiology | en |
dc.title | Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김상완 | - |
dc.contributor.AlternativeAuthor | 허선주 | - |
dc.contributor.AlternativeAuthor | 김성연 | - |
dc.contributor.AlternativeAuthor | 심찬수 | - |
dc.identifier.doi | 10.1016/j.bbrc.2004.12.076 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.