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Dose-specific or dose-dependent effect of growth hormone treatment on the proliferation and differentiation of cultured neuronal cells

Cited 24 time in Web of Science Cited 26 time in Scopus
Authors
Lyuh, Esther; Kim, Hyun-Jung; Kim, Manho; Lee, Jung-Kwon; Park, Kyong-Soo; Yoo, Keun-Young; Lee, Kwang-Woo; Ahn, Yoon-Ok
Issue Date
2007-05-08
Publisher
Elsevier
Citation
Growth Horm IGF Res. 2007 Aug;17(4):315-22. Epub 2007 May 7.
Keywords
AnimalsCell Cycle/drug effectsCell Differentiation/*drug effectsCell Proliferation/*drug effectsCells, CulturedDose-Response Relationship, DrugDrug Evaluation, PreclinicalGrowth Hormone/*pharmacologyMAP Kinase Signaling System/physiologyNeurons/cytology/*drug effectsRats
Abstract
OBJECTIVE: GH controls the proliferation of cartilage, fibroblasts or the differentiation of adipose and muscle tissue. However, the effect of GH on neuronal cells remains unknown. The present study was conducted to determine the proliferative or differentiating effect of GH on the nervous system in vitro. DESIGN: Neuronal hybrid cells (VSC4.1) were cultured with GH. The concentration ranged from 0.134 microg/ml up to 1.34 mg/ml. A cell confluency and MTT assay, cell cycle phase analysis with flow cytometry, extracellular receptor kinase (ERK) phosphorylation and mitogen activated protein kinase (MAPK) inhibitor (PD98050) assays were all performed to determine the effect on proliferation. Differentiation was evaluated by neurite outgrowth and neurofilament expression. Terminally differentiated neurons were stained by Hoechst 33342 for apoptotic nuclear fragmentation by degeneration. Poly-adenosyl ribose polymerase (PARP) expression and its cleavage products were evaluated. RESULTS: Cells at concentrations between 0.134 microg/ml and 1.34 microg/ml of GH proliferated with ERK phosphorylation, which was attenuated by MAPK inhibitors. Proliferation decreased at concentrations higher than 13.4 microg/ml; however, neurite outgrowth was observed at these concentrations. Terminally differentiated cells underwent apoptosis and showed nuclear fragmentation by Hoechst 33342 staining. PARP expression was increased with caspase-3 dependent-cleaved fragment. CONCLUSIONS: Our in vitro data demonstrate that GH exerts dual effects; proliferation with a specific GH dose window, or differentiation in a dose-dependent manner in cultured neuronal hybrid cells.
ISSN
1096-6374 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WG5-4NNPCRH-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=89fd6ef79f9cda708373f899e98efa4c

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17482859

https://hdl.handle.net/10371/22331
DOI
https://doi.org/10.1016/j.ghir.2007.03.002
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College of Medicine/School of Medicine (의과대학/대학원)Neuroscience (뇌신경과학전공)Journal Papers (저널논문_뇌신경과학전공)
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