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Dose-specific or dose-dependent effect of growth hormone treatment on the proliferation and differentiation of cultured neuronal cells

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dc.contributor.authorLyuh, Esther-
dc.contributor.authorKim, Hyun-Jung-
dc.contributor.authorKim, Manho-
dc.contributor.authorLee, Jung-Kwon-
dc.contributor.authorPark, Kyong-Soo-
dc.contributor.authorYoo, Keun-Young-
dc.contributor.authorLee, Kwang-Woo-
dc.contributor.authorAhn, Yoon-Ok-
dc.date.accessioned2009-12-24T07:34:46Z-
dc.date.available2009-12-24T07:34:46Z-
dc.date.issued2007-05-08-
dc.identifier.citationGrowth Horm IGF Res. 2007 Aug;17(4):315-22. Epub 2007 May 7.en
dc.identifier.issn1096-6374 (Print)-
dc.identifier.urihttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WG5-4NNPCRH-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=89fd6ef79f9cda708373f899e98efa4c-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17482859-
dc.identifier.urihttps://hdl.handle.net/10371/22331-
dc.description.abstractOBJECTIVE: GH controls the proliferation of cartilage, fibroblasts or the differentiation of adipose and muscle tissue. However, the effect of GH on neuronal cells remains unknown. The present study was conducted to determine the proliferative or differentiating effect of GH on the nervous system in vitro. DESIGN: Neuronal hybrid cells (VSC4.1) were cultured with GH. The concentration ranged from 0.134 microg/ml up to 1.34 mg/ml. A cell confluency and MTT assay, cell cycle phase analysis with flow cytometry, extracellular receptor kinase (ERK) phosphorylation and mitogen activated protein kinase (MAPK) inhibitor (PD98050) assays were all performed to determine the effect on proliferation. Differentiation was evaluated by neurite outgrowth and neurofilament expression. Terminally differentiated neurons were stained by Hoechst 33342 for apoptotic nuclear fragmentation by degeneration. Poly-adenosyl ribose polymerase (PARP) expression and its cleavage products were evaluated. RESULTS: Cells at concentrations between 0.134 microg/ml and 1.34 microg/ml of GH proliferated with ERK phosphorylation, which was attenuated by MAPK inhibitors. Proliferation decreased at concentrations higher than 13.4 microg/ml; however, neurite outgrowth was observed at these concentrations. Terminally differentiated cells underwent apoptosis and showed nuclear fragmentation by Hoechst 33342 staining. PARP expression was increased with caspase-3 dependent-cleaved fragment. CONCLUSIONS: Our in vitro data demonstrate that GH exerts dual effects; proliferation with a specific GH dose window, or differentiation in a dose-dependent manner in cultured neuronal hybrid cells.en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectAnimalsen
dc.subjectCell Cycle/drug effectsen
dc.subjectCell Differentiation/*drug effectsen
dc.subjectCell Proliferation/*drug effectsen
dc.subjectCells, Cultureden
dc.subjectDose-Response Relationship, Drugen
dc.subjectDrug Evaluation, Preclinicalen
dc.subjectGrowth Hormone/*pharmacologyen
dc.subjectMAP Kinase Signaling System/physiologyen
dc.subjectNeurons/cytology/*drug effectsen
dc.subjectRatsen
dc.titleDose-specific or dose-dependent effect of growth hormone treatment on the proliferation and differentiation of cultured neuronal cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor여에스더-
dc.contributor.AlternativeAuthor김현정-
dc.contributor.AlternativeAuthor김만호-
dc.contributor.AlternativeAuthor이정권-
dc.contributor.AlternativeAuthor박경수-
dc.contributor.AlternativeAuthor유근영-
dc.contributor.AlternativeAuthor이광우-
dc.contributor.AlternativeAuthor안윤옥-
dc.identifier.doi10.1016/j.ghir.2007.03.002-
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