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Prediction of gene expression levels and the role of cis-acting elements in age-related cataract by applying a promoter-based modeling approach

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dc.contributor.authorLim, J. M.-
dc.contributor.authorCho, K. H.-
dc.date.accessioned2009-12-24T11:21:35Z-
dc.date.available2009-12-24T11:21:35Z-
dc.date.issued2005-08-06-
dc.identifier.citationBiotechnol Prog. 2005 Jul-Aug;21(4):1032-7.en
dc.identifier.issn8756-7938 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16080680-
dc.identifier.urihttps://hdl.handle.net/10371/22616-
dc.description.abstractCataract is a dynamical process of lens opacity formation involving many inter- and intracellular regulations, as well as metabolic genes and transcription factors. Using a series of microarray-derived mRNA profiles for human cataractogenesis (Hawse et al. Mol. Vision 2003, 9, 515-537), we develop a promoter-based system-theoretic modeling to demonstrate model-driven prediction of gene expression levels and to identify the role of critical cis-acting elements. In this study, 14 key mRNA expression data from the structural and pathological molecules of age-related cataract samples are used. The first seven genes consist of structural molecules, and the second half of genes are composed of heat shock proteins, filensin, and glutathione peroxidase 3. The presented result demonstrates that mRNA expression levels of structural proteins such as crystallins can be successfully predicted from 5' flanking regulatory DNA sequences. In addition, predicted gene expression levels of heat shock protein, beta-tubulin, and alphaA-crystallin accurately estimate the stimulatory or inhibitory role of distributed cis-acting elements, i.e., c-Myc, GATA-1, GR, NE-E, and Pit-1. Although it is difficult to predict the overall gene expression levels in cataract samples, the present study shows the potential use of promoter-based modeling and prediction of the gene expression levels for age-related cataract.en
dc.language.isoen-
dc.publisherAmerican Chemical Societyen
dc.subject5' Flanking Regionen
dc.subjectAge Factorsen
dc.subjectCataract/*genetics/pathologyen
dc.subject*Gene Expressionen
dc.subjectHeat-Shock Proteins/geneticsen
dc.subjectHumansen
dc.subjectLens, Crystalline/pathologyen
dc.subjectModels, Geneticen
dc.subjectPromoter Regions, Genetic/*geneticsen
dc.subject*Regulatory Sequences, Nucleic Aciden
dc.subjectTubulin/geneticsen
dc.subjectalpha-Crystallin A Chain/geneticsen
dc.titlePrediction of gene expression levels and the role of cis-acting elements in age-related cataract by applying a promoter-based modeling approachen
dc.typeArticleen
dc.contributor.AlternativeAuthor임정민-
dc.contributor.AlternativeAuthor조광현-
dc.identifier.doi10.1021/bp050027s-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Immunology (면역학전공)Journal Papers (저널논문_면역학전공)
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