S-Space College of Medicine/School of Medicine (의과대학/대학원) Immunology (면역학전공) Journal Papers (저널논문_면역학전공)
IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
- Hue, J.; Kim, A.; Song, H.; Choi, I.; Park, H.; Kim, T.; Lee, W. J.; Kang, H.; Cho, D.
- Issue Date
- Immunol Lett. 2005 Jan 31;96(2):211-7.
- Animals; Cell Line, Tumor; DNA, Antisense/genetics/pharmacology; Enzyme Activation; Interleukin-18/genetics/pharmacology/*physiology; Melanoma, Experimental/*metabolism; Mice; RNA, Messenger/genetics/metabolism; Reactive Oxygen Species/*metabolism; Stem Cell Factor/*biosynthesis/genetics; p38 Mitogen-Activated Protein Kinases/*metabolism
- It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-L-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.
- 0165-2478 (Print)
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