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IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
Cited 21 time in
Web of Science
Cited 22 time in Scopus
- Authors
- Issue Date
- 2004-12-09
- Publisher
- Elsevier
- Citation
- Immunol Lett. 2005 Jan 31;96(2):211-7.
- Keywords
- Animals ; Cell Line, Tumor ; DNA, Antisense/genetics/pharmacology ; Enzyme Activation ; Interleukin-18/genetics/pharmacology/*physiology ; Melanoma, Experimental/*metabolism ; Mice ; RNA, Messenger/genetics/metabolism ; Reactive Oxygen Species/*metabolism ; Stem Cell Factor/*biosynthesis/genetics ; p38 Mitogen-Activated Protein Kinases/*metabolism
- Abstract
- It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-L-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.
- ISSN
- 0165-2478 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15585325
https://hdl.handle.net/10371/22640
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