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IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity

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dc.contributor.authorHue, J.-
dc.contributor.authorKim, A.-
dc.contributor.authorSong, H.-
dc.contributor.authorChoi, I.-
dc.contributor.authorPark, H.-
dc.contributor.authorKim, T.-
dc.contributor.authorLee, W. J.-
dc.contributor.authorKang, H.-
dc.contributor.authorCho, D.-
dc.date.accessioned2009-12-24T11:53:06Z-
dc.date.available2009-12-24T11:53:06Z-
dc.date.issued2004-12-09-
dc.identifier.citationImmunol Lett. 2005 Jan 31;96(2):211-7.en
dc.identifier.issn0165-2478 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15585325-
dc.identifier.urihttps://hdl.handle.net/10371/22640-
dc.description.abstractIt has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-L-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectAnimalsen
dc.subjectCell Line, Tumoren
dc.subjectDNA, Antisense/genetics/pharmacologyen
dc.subjectEnzyme Activationen
dc.subjectInterleukin-18/genetics/pharmacology/*physiologyen
dc.subjectMelanoma, Experimental/*metabolismen
dc.subjectMiceen
dc.subjectRNA, Messenger/genetics/metabolismen
dc.subjectReactive Oxygen Species/*metabolismen
dc.subjectStem Cell Factor/*biosynthesis/geneticsen
dc.subjectp38 Mitogen-Activated Protein Kinases/*metabolismen
dc.titleIL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activityen
dc.typeArticleen
dc.contributor.AlternativeAuthor김안근-
dc.contributor.AlternativeAuthor송현근-
dc.contributor.AlternativeAuthor박현정-
dc.contributor.AlternativeAuthor김태성-
dc.contributor.AlternativeAuthor이왕재-
dc.contributor.AlternativeAuthor강형식-
dc.contributor.AlternativeAuthor조대호-
dc.identifier.doi10.1016/j.imlet.2004.08.008-
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