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IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hue, J. | - |
dc.contributor.author | Kim, A. | - |
dc.contributor.author | Song, H. | - |
dc.contributor.author | Choi, I. | - |
dc.contributor.author | Park, H. | - |
dc.contributor.author | Kim, T. | - |
dc.contributor.author | Lee, W. J. | - |
dc.contributor.author | Kang, H. | - |
dc.contributor.author | Cho, D. | - |
dc.date.accessioned | 2009-12-24T11:53:06Z | - |
dc.date.available | 2009-12-24T11:53:06Z | - |
dc.date.issued | 2004-12-09 | - |
dc.identifier.citation | Immunol Lett. 2005 Jan 31;96(2):211-7. | en |
dc.identifier.issn | 0165-2478 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15585325 | - |
dc.identifier.uri | https://hdl.handle.net/10371/22640 | - |
dc.description.abstract | It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-L-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | DNA, Antisense/genetics/pharmacology | en |
dc.subject | Enzyme Activation | en |
dc.subject | Interleukin-18/genetics/pharmacology/*physiology | en |
dc.subject | Melanoma, Experimental/*metabolism | en |
dc.subject | Mice | en |
dc.subject | RNA, Messenger/genetics/metabolism | en |
dc.subject | Reactive Oxygen Species/*metabolism | en |
dc.subject | Stem Cell Factor/*biosynthesis/genetics | en |
dc.subject | p38 Mitogen-Activated Protein Kinases/*metabolism | en |
dc.title | IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김안근 | - |
dc.contributor.AlternativeAuthor | 송현근 | - |
dc.contributor.AlternativeAuthor | 박현정 | - |
dc.contributor.AlternativeAuthor | 김태성 | - |
dc.contributor.AlternativeAuthor | 이왕재 | - |
dc.contributor.AlternativeAuthor | 강형식 | - |
dc.contributor.AlternativeAuthor | 조대호 | - |
dc.identifier.doi | 10.1016/j.imlet.2004.08.008 | - |
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