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Inhibition of proinflammatory cytokine expression by NF-kappaB (p65) antisense oligonucleotide in Helicobacter pylori-infected mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sang Gyun | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Chae, Jung Hyun | - |
dc.contributor.author | Sung, Song In | - |
dc.date.accessioned | 2009-12-28T08:38:17Z | - |
dc.date.available | 2009-12-28T08:38:17Z | - |
dc.date.issued | 2005-11-24 | - |
dc.identifier.citation | Helicobacter. 2005 Dec;10(6):559-66. | en |
dc.identifier.issn | 1083-4389 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16302981 | - |
dc.identifier.uri | https://hdl.handle.net/10371/22865 | - |
dc.description.abstract | BACKGROUND: Helicobacter pylori induces the expression of proinflammatory cytokines in vitro by activating nuclear factor-kappaB, a transcriptional regulator. However, it has not been clarified whether H. pylori-induced proinflammatory cytokines are also mediated through nuclear factor-kappaB in vivo. The aim of this study was to evaluate the role of nuclear factor-kappaB on the expressions of proinflammatory cytokines in H. pylori-infected mice. MATERIALS AND METHODS: We evaluated nuclear factor-kappaB (p65) activation in the H. pylori-infected gastric mucosa of mice by immunofluorescent staining using antip65 polyclonal antibody, and the expressions of proinflammatory cytokines with inhibition of nuclear factor-kappaB pathway by using phosphorothioate antisense and sense oligonucleotide against the nuclear factor-kappaB (p65). RESULTS: In the H. pylori-infected gastric mucosa of mice, immunofluorescent staining using antip65 polyclonal antibody showed nuclear factor-kappaB (p65) activation, which was particularly localized to epithelial cells. Tumor necrosis factor-alpha and interleukin-1beta concentrations in gastric mucosa by enzyme-linked immunosorbent assay (ELISA) were elevated in the infected group versus the uninfected group. Pretreatment with nuclear factor-kappaB (p65) antisense oligonucleotide inhibited the activation of nuclear factor-kappaB and the expressions of tumor necrosis factor-alpha and interleukin-1beta in H. pylori-infected gastric mucosa. Sense oligonucleotide did not influence on the expression of proinflammatory cytokines. CONCLUSIONS: H. pylori infection was found to activate the expressions of proinflammatory cytokines via nuclear factor-kappaB in vivo, and this may play an important role in the initiation of H. pylori-induced gastric inflammation. | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Animals | en |
dc.subject | Cytokines/*antagonists & inhibitors/metabolism | en |
dc.subject | Female | en |
dc.subject | Gastric Mucosa/drug effects/*immunology/microbiology/physiopathology | en |
dc.subject | Helicobacter Infections/*drug therapy/immunology/physiopathology | en |
dc.subject | Helicobacter pylori/*immunology/pathogenicity | en |
dc.subject | Interleukin-1/antagonists & inhibitors/metabolism | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Oligonucleotides, Antisense/administration & | en |
dc.subject | dosage/*pharmacology/therapeutic use | en |
dc.subject | Transcription Factor RelA/*antagonists & inhibitors/metabolism | en |
dc.subject | Tumor Necrosis Factor-alpha/antagonists & inhibitors/metabolism | en |
dc.title | Inhibition of proinflammatory cytokine expression by NF-kappaB (p65) antisense oligonucleotide in Helicobacter pylori-infected mice | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김상균 | - |
dc.contributor.AlternativeAuthor | 김주성 | - |
dc.contributor.AlternativeAuthor | 김정목 | - |
dc.contributor.AlternativeAuthor | 채정현 | - |
dc.contributor.AlternativeAuthor | 성송인 | - |
dc.identifier.doi | 10.1111/j.1523-5378.2005.00365.x | - |
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