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Direct inhibition of a PKA inhibitor, H-89 on KV channels in rabbit coronary arterial smooth muscle cells

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dc.contributor.authorSon, Youn Kyoung-
dc.contributor.authorPark, Won Sun-
dc.contributor.authorKim, Sung Joon-
dc.contributor.authorEarm, Yung E.-
dc.contributor.authorKim, Nari-
dc.contributor.authorYoum, Jae Boum-
dc.contributor.authorWarda, Mohamad-
dc.contributor.authorKim, Euiyong-
dc.contributor.authorHan, Jin-
dc.date.accessioned2009-12-30-
dc.date.available2009-12-30-
dc.date.issued2006-02-04-
dc.identifier.citationBiochem Biophys Res Commun. 2006 Mar 24;341(4):931-7. Epub 2006 Jan 23.en
dc.identifier.issn0006-291X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16455049-
dc.identifier.urihttps://hdl.handle.net/10371/23380-
dc.description.abstractWe examined the effects of the protein kinase A (PKA) inhibitor H-89 on voltage-dependent K(+) (K(V)) currents in freshly isolated rabbit coronary arterial smooth muscle cells, using a whole-cell patch clamp technique. H-89 inhibited the K(V) current in a concentration-dependent manner, with a K(d) value of 1.02 microM. However, the PKA inhibitors KT 5720 and Rp-8-CPT-cAMPS did not significantly alter the K(V) current or the inhibitory effects of H-89 on the K(V) current. Moreover, H-85, a structurally similar but inactive analog of H-89, showed similar inhibitory effects on the K(V) channel. H-89 had no effect on the voltage-dependency of activation or inactivation, or on recovery kinetics. These results suggest that in rabbit coronary arterial smooth muscle cells, H-89 inhibits the K(V) current directly by blocking the pore cavity, an effect independent of PKA inhibition.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectAnimalsen
dc.subjectCarbazoles/pharmacologyen
dc.subjectCoronary Vessels/cytology/drug effectsen
dc.subjectCyclic AMP/analogs & derivatives/pharmacologyen
dc.subjectCyclic AMP-Dependent Protein Kinases/*antagonists & inhibitorsen
dc.subjectFemaleen
dc.subjectIndoles/pharmacologyen
dc.subjectIsoquinolines/*pharmacologyen
dc.subjectMaleen
dc.subjectMuscle, Smooth, Vascular/*cytology/drug effectsen
dc.subjectPotassium Channels, Voltage-Gated/*drug effectsen
dc.subjectPyrroles/pharmacologyen
dc.subjectRabbitsen
dc.subjectSulfonamides/*pharmacologyen
dc.subjectThionucleotides/pharmacologyen
dc.titleDirect inhibition of a PKA inhibitor, H-89 on KV channels in rabbit coronary arterial smooth muscle cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor손윤경-
dc.contributor.AlternativeAuthor박원선-
dc.contributor.AlternativeAuthor김성준-
dc.contributor.AlternativeAuthor엄융의-
dc.contributor.AlternativeAuthor김나리-
dc.contributor.AlternativeAuthor염재범-
dc.contributor.AlternativeAuthor김의용-
dc.contributor.AlternativeAuthor한진-
dc.identifier.doi10.1016/j.bbrc.2006.01.053-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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