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Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation
DC Field | Value | Language |
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dc.contributor.author | Son, Yeon Sung | - |
dc.contributor.author | Park, Jae Hyun | - |
dc.contributor.author | Kang, Young Kook | - |
dc.contributor.author | Park, Jin-Sung | - |
dc.contributor.author | Choi, Hong Seo | - |
dc.contributor.author | Lim, Ji Young | - |
dc.contributor.author | Lee, Jeoung Eun | - |
dc.contributor.author | Lee, Jung Bok | - |
dc.contributor.author | Ko, Myoung Seok | - |
dc.contributor.author | Kim, Yong-Sam | - |
dc.contributor.author | Ko, Jeong-Heon | - |
dc.contributor.author | Yoon, Hyun Soo | - |
dc.contributor.author | Lee, Kwang-Woong | - |
dc.contributor.author | Seong, Rho Hyun | - |
dc.contributor.author | Moon, Shin Yong | - |
dc.contributor.author | Ryu, Chun Jeih | - |
dc.contributor.author | Hong, Hyo Jeong | - |
dc.date.accessioned | 2009-12-30 | - |
dc.date.available | 2009-12-30 | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Stem Cells 2005;23:1502-1513 | en |
dc.identifier.issn | 1066-5099 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16100000 | - |
dc.identifier.uri | https://hdl.handle.net/10371/23394 | - |
dc.description.abstract | The cell-surface markers used routinely to define the undifferentiated state and pluripotency of human embryonic stem cells (hESCs) are those used in mouse embryonic stem cells (mESCs) because of a lack of markers directly originated from hESC itself. To identify more hESC-specific cell-surface markers, we generated a panel of monoclonal antibodies (MAbs) by immunizing the irradiated cell clumps of hESC line Miz-hES1, and selected 26 MAbs that were able to bind to Miz-hES1 cells but not to mESCs, mouse embryonic fibroblast cells, and STO cells. Most antibodies did not bind to human neural progenitor cells derived from the Miz-hES1 cells, either. Of these, MAb 20-202S (IgG1, kappa) immunoprecipitated a cell-surface protein of 72-kDa from the lysate of biotin-labeled Miz-hES1 cells, which was identified to be heat shock 70-kDa protein 8 isoform 1 (HSPA8) by quadrupole time-of-flight tandem mass spectrometry. Immunocytochemical analyses proved that the HSPA8 protein was also present on the surface of hESC lines Miz-hES4, Miz-hES6, and HSF6. Two-color flow cytometric analysis of Miz-hES1 and HSF6 showed the coexpression of the HSPA8 protein with other hESC markers such as stage-specific embryonic antigen 3 (SSEA3), SSEA4, TRA-1-60, and TRA-1-81. Flow cytometric and Western blot analyses using various cells showed that MAb 20-202S specifically bound to the HSPA8 protein on the surface of Miz-hES1, contrary to other anti-HSP70 antibodies examined. Furthermore, the surface expression of the HSPA8 protein on Miz-hES1 was markedly downregulated upon differentiation. These data indicate that a novel MAb 20-202S recognizes the HSPA8 protein on the surface of hESCs and suggest that the HSPA8 protein is a putative cell-surface marker for undifferentiated hESCs. | en |
dc.language.iso | en | en |
dc.publisher | AlphaMed Press | en |
dc.subject | Antibodies, Monoclonal/metabolism | en |
dc.subject | Antigens, Surface/metabolism | en |
dc.subject | Cell Line | en |
dc.subject | Embryo Research | en |
dc.subject | HSP70 Heat-Shock Proteins/*biosynthesis | en |
dc.subject | Hela Cells | en |
dc.subject | Humans | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Mass Spectrometry | en |
dc.subject | Protein Isoforms/*biosynthesis | en |
dc.subject | Stem Cells/cytology/*metabolism | en |
dc.subject | Cell Differentiation | - |
dc.subject | Down-Regulation | - |
dc.title | Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 손연성 | - |
dc.contributor.AlternativeAuthor | 박재현 | - |
dc.contributor.AlternativeAuthor | 강영국 | - |
dc.contributor.AlternativeAuthor | 박진성 | - |
dc.contributor.AlternativeAuthor | 최홍서 | - |
dc.contributor.AlternativeAuthor | 임지영 | - |
dc.contributor.AlternativeAuthor | 이정은 | - |
dc.contributor.AlternativeAuthor | 이정복 | - |
dc.contributor.AlternativeAuthor | 고명석 | - |
dc.contributor.AlternativeAuthor | 김용삼 | - |
dc.contributor.AlternativeAuthor | 고정현 | - |
dc.contributor.AlternativeAuthor | 윤현수 | - |
dc.contributor.AlternativeAuthor | 이광웅 | - |
dc.contributor.AlternativeAuthor | 성노현 | - |
dc.contributor.AlternativeAuthor | 문신용 | - |
dc.contributor.AlternativeAuthor | 홍효정 | - |
dc.identifier.doi | 10.1634/stemcells.2004-0307 | - |
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