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Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation

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dc.contributor.authorSon, Yeon Sung-
dc.contributor.authorPark, Jae Hyun-
dc.contributor.authorKang, Young Kook-
dc.contributor.authorPark, Jin-Sung-
dc.contributor.authorChoi, Hong Seo-
dc.contributor.authorLim, Ji Young-
dc.contributor.authorLee, Jeoung Eun-
dc.contributor.authorLee, Jung Bok-
dc.contributor.authorKo, Myoung Seok-
dc.contributor.authorKim, Yong-Sam-
dc.contributor.authorKo, Jeong-Heon-
dc.contributor.authorYoon, Hyun Soo-
dc.contributor.authorLee, Kwang-Woong-
dc.contributor.authorSeong, Rho Hyun-
dc.contributor.authorMoon, Shin Yong-
dc.contributor.authorRyu, Chun Jeih-
dc.contributor.authorHong, Hyo Jeong-
dc.date.accessioned2009-12-30-
dc.date.available2009-12-30-
dc.date.issued2005-
dc.identifier.citationStem Cells 2005;23:1502-1513en
dc.identifier.issn1066-5099 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16100000-
dc.identifier.urihttp://hdl.handle.net/10371/23394-
dc.description.abstractThe cell-surface markers used routinely to define the undifferentiated state and pluripotency of human embryonic stem cells (hESCs) are those used in mouse embryonic stem cells (mESCs) because of a lack of markers directly originated from hESC itself. To identify more hESC-specific cell-surface markers, we generated a panel of monoclonal antibodies (MAbs) by immunizing the irradiated cell clumps of hESC line Miz-hES1, and selected 26 MAbs that were able to bind to Miz-hES1 cells but not to mESCs, mouse embryonic fibroblast cells, and STO cells. Most antibodies did not bind to human neural progenitor cells derived from the Miz-hES1 cells, either. Of these, MAb 20-202S (IgG1, kappa) immunoprecipitated a cell-surface protein of 72-kDa from the lysate of biotin-labeled Miz-hES1 cells, which was identified to be heat shock 70-kDa protein 8 isoform 1 (HSPA8) by quadrupole time-of-flight tandem mass spectrometry. Immunocytochemical analyses proved that the HSPA8 protein was also present on the surface of hESC lines Miz-hES4, Miz-hES6, and HSF6. Two-color flow cytometric analysis of Miz-hES1 and HSF6 showed the coexpression of the HSPA8 protein with other hESC markers such as stage-specific embryonic antigen 3 (SSEA3), SSEA4, TRA-1-60, and TRA-1-81. Flow cytometric and Western blot analyses using various cells showed that MAb 20-202S specifically bound to the HSPA8 protein on the surface of Miz-hES1, contrary to other anti-HSP70 antibodies examined. Furthermore, the surface expression of the HSPA8 protein on Miz-hES1 was markedly downregulated upon differentiation. These data indicate that a novel MAb 20-202S recognizes the HSPA8 protein on the surface of hESCs and suggest that the HSPA8 protein is a putative cell-surface marker for undifferentiated hESCs.en
dc.language.isoenen
dc.publisherAlphaMed Pressen
dc.subjectAntibodies, Monoclonal/metabolismen
dc.subjectAntigens, Surface/metabolismen
dc.subject*Cell Differentiationen
dc.subjectCell Lineen
dc.subject*Down-Regulationen
dc.subjectEmbryo Researchen
dc.subjectHSP70 Heat-Shock Proteins/*biosynthesisen
dc.subjectHela Cellsen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectMass Spectrometryen
dc.subjectProtein Isoforms/*biosynthesisen
dc.subjectStem Cells/cytology/*metabolismen
dc.titleHeat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiationen
dc.typeArticleen
dc.contributor.AlternativeAuthor손연성-
dc.contributor.AlternativeAuthor박재현-
dc.contributor.AlternativeAuthor강영국-
dc.contributor.AlternativeAuthor박진성-
dc.contributor.AlternativeAuthor최홍서-
dc.contributor.AlternativeAuthor임지영-
dc.contributor.AlternativeAuthor이정은-
dc.contributor.AlternativeAuthor이정복-
dc.contributor.AlternativeAuthor고명석-
dc.contributor.AlternativeAuthor김용삼-
dc.contributor.AlternativeAuthor고정현-
dc.contributor.AlternativeAuthor윤현수-
dc.contributor.AlternativeAuthor이광웅-
dc.contributor.AlternativeAuthor성노현-
dc.contributor.AlternativeAuthor문신용-
dc.contributor.AlternativeAuthor홍효정-
dc.identifier.doi10.1634/stemcells.2004-0307-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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