Publications
Detailed Information
The anti-cancer effect of COX-2 inhibitors on gastric cancer cells
Cited 34 time in
Web of Science
Cited 36 time in Scopus
- Authors
- Issue Date
- 2007-03-30
- Publisher
- Springer Verlag
- Citation
- Dig Dis Sci. 2007 Jul;52(7):1713-21. Epub 2007 Mar 28.
- Keywords
- Adenocarcinoma/*drug therapy/metabolism ; Apoptosis/drug effects ; Cell Cycle/drug effects ; Cell Line, Tumor ; Cyclooxygenase 2 Inhibitors/pharmacology/*therapeutic use ; Dinoprostone/metabolism ; Humans ; Microscopy, Fluorescence ; Pyrazoles/pharmacology/*therapeutic use ; Stomach Neoplasms/*drug therapy/metabolism ; Sulfonamides/pharmacology/*therapeutic use ; Tetrazolium Salts/diagnostic use ; Thiazoles/diagnostic use
- Abstract
- Epidemiologic studies have shown that nonsteroidal anti-inflammatory drugs could reduce the risk of cancer development including gastric cancer. This study was performed to identify the antineoplastic mechanism in gastric cancer cells affected by celecoxib, a selective COX-2 inhibitor. MTT assay, ELISA for prostaglandin E(2) (PGE(2)), cell-cycle analyses, immunofluorescent staining, and flow cytometry were performed after treating human gastric cancer cell lines (AGS and MKN-45) with celecoxib or indomethacin. The viabilities of celecoxib-treated cells decreased in a dose- and time-dependent manner compared with indomethacin. Drop of PGE(2) levels was more prominent in the presence of indomethacin than in that of celecoxib. Celecoxib arrested the cell cycle in the G(0)-G(1) phase, which reduced cell numbers in the S phase. Moreover, celecoxib increased the apoptotic cell proportions, a 4-fold increase over control cells. The anticancer effects of celecoxib on gastric cancer cells appear to be mediated by cell-cycle arrest and apoptosis, and not by COX-2 or PGE(2) suppression alone.
- ISSN
- 0163-2116 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17393325
https://hdl.handle.net/10371/23422
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.