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Propofol reverses oxidative stress-attenuated glutamate transporter EAAT3 activity: evidence of protein kinase C involvement
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Jung-Yeon | - |
dc.contributor.author | Park, Kum-Suk | - |
dc.contributor.author | Kim, Jin-Hee | - |
dc.contributor.author | Do, Sang-Hwan | - |
dc.contributor.author | Zuo, Zhiyi | - |
dc.date.accessioned | 2009-12-31T04:11:43Z | - |
dc.date.available | 2009-12-31T04:11:43Z | - |
dc.date.issued | 2007-03-27 | - |
dc.identifier.citation | Eur J Pharmacol. 2007 Jun 22;565(1-3):83-8. Epub 2007 Mar 3. | en |
dc.identifier.issn | 0014-2999 (Print) | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1J-4N5TNBT-6&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=79300f490206700a8fb1b3e50da73919 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17382927 | - |
dc.identifier.uri | https://hdl.handle.net/10371/24236 | - |
dc.description.abstract | The authors investigated the effects of propofol on EAAT3 (excitatory amino acid transporter 3) activity under oxidative stress induced by tert-butyl hydroperoxide (t-BHP), and the mediation of these effects by protein kinase C (PKC). Rat EAAT3 was expressed in Xenopus oocytes and L-glutamate (30 microM)-induced membrane currents were measured using the two-electrode voltage clamp technique. Exposure of these oocytes to t-BHP (1-20 mM) for 10 min dose-dependently decreased EAAT3 activity, and t-BHP (5 mM) significantly decreased the Vmax, but not the Km of EAAT3 for glutamate, and propofol (1-100 microM) dose-dependently reversed this t-BHP-attenuated EAAT3 activity. Phorbol-12-myristate-13-acetate (a PKC activator), also abolished this t-BHP-induced reduction in EAAT3 activity, whereas staurosporine (a PKC inhibitor), significantly decreased EAAT3 activity. However, as compared with staurosporine or t-BHP alone, t-BHP and staurosporine in combination did not further reduce EAAT3 activity. A similar pattern was observed for chelerythrine (also a PKC inhibitor). In oocytes pretreated with combinations of t-BHP and PMA (or staurosporine), propofol failed to change EAAT3 activity. Our results suggest that propofol restores oxidative stress-reduced EAAT3 activity and that these effects of propofol may be PKC-mediated. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Dose-Response Relationship, Drug | en |
dc.subject | Excitatory Amino Acid Transporter 3/*drug effects/genetics/physiology | en |
dc.subject | Neuroprotective Agents/pharmacology | en |
dc.subject | Propofol/*pharmacology | en |
dc.subject | Protein Kinase C/*physiology | en |
dc.subject | Rats | en |
dc.subject | Staurosporine/pharmacology | en |
dc.subject | Tetradecanoylphorbol Acetate/pharmacology | en |
dc.subject | Xenopus | en |
dc.subject | tert-Butylhydroperoxide/pharmacology | en |
dc.subject | Oxidative Stress | - |
dc.title | Propofol reverses oxidative stress-attenuated glutamate transporter EAAT3 activity: evidence of protein kinase C involvement | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 윤정연 | - |
dc.contributor.AlternativeAuthor | 박금숙 | - |
dc.contributor.AlternativeAuthor | 김진희 | - |
dc.contributor.AlternativeAuthor | 도상환 | - |
dc.identifier.doi | 10.1016/j.ejphar.2007.02.045 | - |
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