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Bradykinin modulates pacemaker currents through bradykinin B2 receptors in cultured interstitial cells of Cajal from the murine small intestine

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dc.contributor.authorChoi, Seok-
dc.contributor.authorPark, Do Young-
dc.contributor.authorYeum, Cheol Ho-
dc.contributor.authorChang, In Youb-
dc.contributor.authorYou, Ho Jin-
dc.contributor.authorPark, Chan Guk-
dc.contributor.authorKim, Man Yoo-
dc.contributor.authorKong, In Deok-
dc.contributor.authorSo, Insuk-
dc.contributor.authorKim, Ki Whan-
dc.contributor.authorJun, Jae Yeoul-
dc.date.accessioned2010-01-07T02:20:36Z-
dc.date.available2010-01-07T02:20:36Z-
dc.date.issued2006-06-20-
dc.identifier.citationBr J Pharmacol. 2006 Aug;148(7):918-26. Epub 2006 Jun 19.en
dc.identifier.govdochttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16783409-
dc.identifier.issn0007-1188 (Print)-
dc.identifier.urihttp://hdl.handle.net/10371/27852-
dc.description.abstractWe studied the modulation of pacemaker activities by bradykinin in cultured interstitial cells of Cajal (ICC) from murine small intestine with the whole-cell patch-clamp technique. Externally applied bradykinin produced membrane depolarization in the current-clamp mode and increased tonic inward pacemaker currents in the voltage-clamp mode. Pretreatment with bradykinin B1 antagonist did not block the bradykinin-induced effects on pacemaker currents. However, pretreatment with bradykinin B2 antagonist selectively blocked the bradykinin-induced effects. Also, only externally applied selective bradykinin B2 receptor agonist produced tonic inward pacemaker currents and ICC revealed a colocalization of the bradykinin B2 receptor and c-kit immunoreactivities, but bradykinin B1 receptors did not localize in ICC. External Na(+)-free solution abolished the generation of pacemaker currents and inhibited the bradykinin-induced tonic inward current. However, a Cl(-) channel blocker (DIDS) did not block the bradykinin-induced tonic inward current. The pretreatment with Ca(2+)-free solution and thapsigargin, a Ca(2+)-ATPase inhibitor in endoplasmic reticulum, abolished the generation of pacemaker currents and suppressed the bradykinin-induced action.Chelerythrine and calphostin C, protein kinase C inhibitors or naproxen, an inhibitor of cyclooxygenase, did not block the bradykinin-induced effects on pacemaker currents. These results suggest that bradykinin modulates the pacemaker activities through bradykinin B2 receptor activation in ICC by external Ca(2+) influx and internal Ca(2+) release via protein kinase C- or cyclooxygenase-independent mechanism. Therefore, the ICC are targets for bradykinin and their interaction can affect intestinal motility.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.subjectAnimalsen
dc.subjectBiological Clocks/*drug effectsen
dc.subjectBradykinin/*pharmacologyen
dc.subjectCalcium/physiologyen
dc.subjectCalcium-Transporting ATPases/antagonists & inhibitorsen
dc.subjectCells, Cultureden
dc.subjectChloride Channels/antagonists & inhibitorsen
dc.subjectCyclooxygenase Inhibitors/pharmacologyen
dc.subjectEnzyme Inhibitors/pharmacologyen
dc.subjectFemaleen
dc.subjectIntestine, Small/cytology/drug effects/*metabolismen
dc.subjectImmunohistochemistryen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectPatch-Clamp Techniquesen
dc.subjectProtein Kinase C/antagonists & inhibitorsen
dc.subjectReceptor, Bradykinin B2/*drug effectsen
dc.subjectSignal Transduction/drug effectsen
dc.subjectSodium/physiologyen
dc.titleBradykinin modulates pacemaker currents through bradykinin B2 receptors in cultured interstitial cells of Cajal from the murine small intestineen
dc.typeArticleen
dc.contributor.AlternativeAuthor최석-
dc.contributor.AlternativeAuthor박도영-
dc.contributor.AlternativeAuthor염철호-
dc.contributor.AlternativeAuthor장인엽-
dc.contributor.AlternativeAuthor유호진-
dc.contributor.AlternativeAuthor박찬국-
dc.contributor.AlternativeAuthor김만유-
dc.contributor.AlternativeAuthor공인덕-
dc.contributor.AlternativeAuthor소인숙-
dc.contributor.AlternativeAuthor김기환-
dc.contributor.AlternativeAuthor전제열-
dc.identifier.doi10.1038/sj.bjp.0706806-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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