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In vivo imaging of adenovirus transduction and enhanced therapeutic efficacy of combination therapy with conditionally replicating adenovirus and adenovirus-p27
DC Field | Value | Language |
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dc.contributor.author | Lee, Choon-Taek | - |
dc.contributor.author | Lee, Yoon-Jin | - |
dc.contributor.author | Kwon, Sung-Youn | - |
dc.contributor.author | Lee, Jaeho | - |
dc.contributor.author | Kim, Kwang Il | - |
dc.contributor.author | Park, Kyung-Ho | - |
dc.contributor.author | Kang, Joo Hyun | - |
dc.contributor.author | Yoo, Chul-Gyu | - |
dc.contributor.author | Kim, Young Whan | - |
dc.contributor.author | Han, Sung Koo | - |
dc.contributor.author | Chung, June-Key | - |
dc.contributor.author | Shim, Young-Soo | - |
dc.contributor.author | Curiel, David T | - |
dc.contributor.author | Carbone, David P | - |
dc.date.accessioned | 2010-01-07T05:18:42Z | - |
dc.date.available | 2010-01-07T05:18:42Z | - |
dc.date.issued | 2006-01-07 | - |
dc.identifier.citation | Cancer Res. 2006 Jan 1;66(1):372-7. | en |
dc.identifier.issn | 0008-5472 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16397251 | - |
dc.identifier.uri | https://hdl.handle.net/10371/28252 | - |
dc.description.abstract | Gene therapy is hampered by poor gene transfer to the tumor mass. We previously proposed a combination adenoviral gene therapy containing a conditionally replicating adenovirus (CRAD) expressing mutant E1 (delta24RGD) and a replication-defective E1-deleted adenovirus to enhance the efficiency of gene transfer. Mutant E1 expressed by delta24RGD enables the replication of replication-defective adenoviruses in tumors when cancer cells are co-infected with both viruses. In this study, gene transfer rates in xenografts tumors were monitored by bioluminescence in cells infected with the replication-defective adenovirus-luciferase (ad-luc). Tumor masses treated with CRAD + ad-luc showed dramatically stronger and more prolonged luciferase expression than ad-luc-treated tumors and this expression spread through the entire tumor mass without significant systemic spread. Transduction with CRAD + replication-defective adenovirus-p27 increased the expression of p27 by 24-fold versus transduction with ad-p27 alone. Treatment of a lung cancer cell line and of established lung cancer xenografts with CRAD + adenovirus-p27 also induced stronger growth suppression than treatment with either virus alone. These findings confirm the selective replication of E1-deleted adenovirus containing a therapeutic gene due to the presence of mutant E1 produced by delta24RGD in tumors. Moreover, this replication increased the therapeutic gene transfer rate and enhanced its antitumor effects. | en |
dc.language.iso | en | - |
dc.publisher | American Association for Cancer Research | en |
dc.subject | Adenoviridae/genetics/*physiology | en |
dc.subject | Animals | en |
dc.subject | Cyclin-Dependent Kinase Inhibitor p27/biosynthesis/*genetics | en |
dc.subject | Gene Expression | en |
dc.subject | Gene Therapy/*methods | en |
dc.subject | Humans | en |
dc.subject | Luciferases/biosynthesis/genetics | en |
dc.subject | Luminescent Measurements | en |
dc.subject | Lung Neoplasms/genetics/metabolism/*therapy/virology | en |
dc.subject | Mice | en |
dc.subject | Transduction, Genetic | en |
dc.subject | Virus Replication | en |
dc.subject | Xenograft Model Antitumor Assays | en |
dc.title | In vivo imaging of adenovirus transduction and enhanced therapeutic efficacy of combination therapy with conditionally replicating adenovirus and adenovirus-p27 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이춘택 | - |
dc.contributor.AlternativeAuthor | 이윤진 | - |
dc.contributor.AlternativeAuthor | 권성연 | - |
dc.contributor.AlternativeAuthor | 이재호 | - |
dc.contributor.AlternativeAuthor | 김광일 | - |
dc.contributor.AlternativeAuthor | 박경호 | - |
dc.contributor.AlternativeAuthor | 강주현 | - |
dc.contributor.AlternativeAuthor | 유철규 | - |
dc.contributor.AlternativeAuthor | 김영환 | - |
dc.contributor.AlternativeAuthor | 한성구 | - |
dc.contributor.AlternativeAuthor | 정준기 | - |
dc.contributor.AlternativeAuthor | 심영수 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-05-1515 | - |
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