S-Space College of Medicine/School of Medicine (의과대학/대학원) Microbiology (미생물학전공) Journal Papers (저널논문_미생물학전공)
Characterization of two hepatitis B virus populations in a single Korean hepatocellular carcinoma patient with an HBeAg-negative serostatus: a novel X-Gene-deleted strain with inverted duplication sequences of upstream enhancer site II
- Issue Date
- Intervirology. 2007;50(4):273-80. Epub 2007 Jun 15.
- Carcinoma, Hepatocellular/*virology ; Enhancer Elements, Genetic/*genetics ; Evolution, Molecular ; Gene Deletion ; Gene Duplication ; Hepatitis B e Antigens/*blood ; Hepatitis B virus/*classification/genetics/isolation & ; purification/pathogenicity ; Humans ; Liver Neoplasms/*virology ; Male ; Middle Aged ; Molecular Sequence Data ; Recombination, Genetic ; Sequence Analysis, DNA ; Trans-Activators/*genetics ; Genome, Viral
- OBJECTIVES: The aim of the study was to elucidate mutation patterns related to hepatocarcinogenesis in a Korean hepatocellular carcinoma (HCC) patient. METHODS: We analyzed full genome sequences of 6 hepatitis B virus (HBV) clones from an HCC patient. RESULTS: This patient harbored 2 HBV populations with genomes of different lengths (3,221 and 2,212 bp). In addition, we found 2 characteristic features not described so far in the full-genome sequence of deleted strains. First, 3 large deletion events (847, 144 and 48 bp) and a premature termination of the 182th codon of the surface antigen could lead to truncated or possibly nonfunctional forms of all HBV proteins. Second, these showed a novel mutation type not reported to date, which is a complex of an inverted duplication of 36-bp sequences containing an upstream enhancer site II (UEII), a remote insertion, and a large deletion event of the X region by homologous recombination. CONCLUSION: The fact that UEII is a binding site of liver-specific nuclear factor, which is expressed only in highly differentiated liver cells such as cancerous HepG2, strongly suggests a relationship between this novel mutation and hepatocarcinogenesis in this patient.
- 1423-0100 (Electronic)
- Files in This Item: There are no files associated with this item.