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Characterization of two hepatitis B virus populations in a single Korean hepatocellular carcinoma patient with an HBeAg-negative serostatus: a novel X-Gene-deleted strain with inverted duplication sequences of upstream enhancer site II
Cited 13 time in
Web of Science
Cited 14 time in Scopus
- Authors
- Issue Date
- 2007-06-16
- Publisher
- Karger
- Citation
- Intervirology. 2007;50(4):273-80. Epub 2007 Jun 15.
- Keywords
- Carcinoma, Hepatocellular/*virology ; Enhancer Elements, Genetic/*genetics ; Evolution, Molecular ; Gene Deletion ; Gene Duplication ; Hepatitis B e Antigens/*blood ; Hepatitis B virus/*classification/genetics/isolation & ; purification/pathogenicity ; Humans ; Liver Neoplasms/*virology ; Male ; Middle Aged ; Molecular Sequence Data ; Recombination, Genetic ; Sequence Analysis, DNA ; Trans-Activators/*genetics ; Genome, Viral
- Abstract
- OBJECTIVES: The aim of the study was to elucidate mutation patterns related to hepatocarcinogenesis in a Korean hepatocellular carcinoma (HCC) patient. METHODS: We analyzed full genome sequences of 6 hepatitis B virus (HBV) clones from an HCC patient. RESULTS: This patient harbored 2 HBV populations with genomes of different lengths (3,221 and 2,212 bp). In addition, we found 2 characteristic features not described so far in the full-genome sequence of deleted strains. First, 3 large deletion events (847, 144 and 48 bp) and a premature termination of the 182th codon of the surface antigen could lead to truncated or possibly nonfunctional forms of all HBV proteins. Second, these showed a novel mutation type not reported to date, which is a complex of an inverted duplication of 36-bp sequences containing an upstream enhancer site II (UEII), a remote insertion, and a large deletion event of the X region by homologous recombination. CONCLUSION: The fact that UEII is a binding site of liver-specific nuclear factor, which is expressed only in highly differentiated liver cells such as cancerous HepG2, strongly suggests a relationship between this novel mutation and hepatocarcinogenesis in this patient.
- ISSN
- 1423-0100 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17570929
https://hdl.handle.net/10371/28329
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