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Diagnostic usefulness of a T-cell based assay for extrapulmonary tuberculosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sung-Han | - |
dc.contributor.author | Choi, Su-Jin | - |
dc.contributor.author | Kim, Hong-Bin | - |
dc.contributor.author | Kim, Nam-Joong | - |
dc.contributor.author | Oh, Myoung-Don | - |
dc.contributor.author | Choe, Kang-Won | - |
dc.date.accessioned | 2010-01-12T02:00:27Z | - |
dc.date.available | 2010-01-12T02:00:27Z | - |
dc.date.issued | 2007-11-14 | - |
dc.identifier.citation | Arch Intern Med. 2007 Nov 12;167(20):2255-9. | en |
dc.identifier.issn | 0003-9926 (Print) | - |
dc.identifier.uri | http://archinte.ama-assn.org/cgi/content/abstract/167/20/2255 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17998500 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29552 | - |
dc.description.abstract | BACKGROUND: Diagnosing extrapulmonary tuberculosis (E-TB) remains a challenge. A recently developed Mycobacterium tuberculosis-specific region of difference 1 gene-based assay for diagnosing tuberculosis infection showed promising results. However, the diagnostic usefulness of this assay remains to be determined compared with tuberculin skin test (TST) in patients with suspected E-TB in clinical practice. METHODS: All patients with suspected E-TB were prospectively enrolled in a tertiary care hospital during a 9-month period. In addition to the conventional tests for diagnosing E-TB, the interferon gamma-producing T-cell responses to early secreted antigenic target 6 and culture filtrate protein 10 by enzyme-linked immunospot (ELISPOT) assay were performed. Final diagnosis in patients having suspected E-TB was classified by clinical category. RESULTS: Seventy-two patients with suspected E-TB were enrolled; 34 (47%) had immunosuppressive conditions. Of 72 patients, 32 (44%) were classified as having E-TB, including 22 with confirmed tuberculosis and 10 with probable tuberculosis, and 35 (49%) were classified as not having tuberculosis. The remaining 5 (7%) had possible tuberculosis and were excluded from the final analysis. Chronic caseating granulomas, acid-fast bacilli stain, M tuberculosis polymerase chain reaction, and cultures for M tuberculosis were positive in 22 (69%), 5 (16%), 15 (47%), and 18 (56%), respectively, of 32 patients with E-TB. The sensitivity and specificity of the TST (induration size, > or =10 mm) were 47% (95% confidence interval [CI], 29%-65%) and 86% (95% CI, 70%-95%), respectively. By comparison, the sensitivity and specificity of the ELISPOT assay were 94% (95% CI, 79%-99%; P < .001 between TST and ELISPOT) and 88% (95% CI, 72%-97%; P =.99 between TST and ELISPOT), respectively. CONCLUSION: The ELISPOT assay is a useful adjunct test for diagnosing E-TB. | en |
dc.language.iso | en | en |
dc.publisher | American Medical Association | en |
dc.subject | Adult | en |
dc.subject | Aged | en |
dc.subject | Antigens, Bacterial/diagnostic use | en |
dc.subject | Bacterial Proteins/diagnostic use | en |
dc.subject | Female | en |
dc.subject | Humans | en |
dc.subject | Male | en |
dc.subject | Middle Aged | en |
dc.subject | Sensitivity and Specificity | en |
dc.subject | T-Lymphocytes/*immunology | en |
dc.subject | Tuberculosis/*diagnosis | en |
dc.subject | Enzyme-Linked Immunosorbent Assay | - |
dc.subject | Tuberculin Test | - |
dc.title | Diagnostic usefulness of a T-cell based assay for extrapulmonary tuberculosis | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김성한 | - |
dc.contributor.AlternativeAuthor | 최수진 | - |
dc.contributor.AlternativeAuthor | 김홍빈 | - |
dc.contributor.AlternativeAuthor | 김남중 | - |
dc.contributor.AlternativeAuthor | 오명돈 | - |
dc.contributor.AlternativeAuthor | 최강원 | - |
dc.identifier.doi | 10.1001/archinte.167.20.2255 | - |
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