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ATP6V0C competes with von Hippel-Lindau protein in hypoxia-inducible factor 1alpha (HIF-1alpha) binding and mediates HIF-1alpha expression by bafilomycin A1

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dc.contributor.authorLim, Ji-Hong-
dc.contributor.authorPark, Jong-Wan-
dc.contributor.authorKim, Sung Joon-
dc.contributor.authorKim, Myung-Suk-
dc.contributor.authorPark, Sang-Ki-
dc.contributor.authorJohnson, Randall S.-
dc.contributor.authorChun, Yang-Sook-
dc.date.accessioned2010-01-12T02:25:01Z-
dc.date.available2010-01-12T02:25:01Z-
dc.date.issued2006-12-21-
dc.identifier.citationMol Pharmacol. 2007 Mar;71(3):942-8. Epub 2006 Dec 18.en
dc.identifier.issn0026-895X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17178925-
dc.identifier.urihttps://hdl.handle.net/10371/29598-
dc.description.abstractHIF-1alpha not only enables cells to survive under hypoxic conditions but also promotes cell cycle arrest and apoptosis. Therefore, its expression should be controlled at optimal levels in growing tumors. We recently reported that bafilomycin A1 exorbitantly expressed HIF-1alpha and induced the p21(WAF1/Cip1)-mediated growth arrest of tumors (Mol Pharmacol 70:1856-1865, 2006). In the present study, we addressed the mechanism underlying bafilomycin-induced HIF-1alpha expression. Bafilomycin stabilized HIF-1alpha under normoxic conditions without changes in intracellular pH. However, when ATP6V0C, the target protein of bafilomycin, was knocked down, this bafilomycin effect was significantly attenuated. Inversely, ATP6V0C expression increased HIF-1alpha levels in a gene dose-dependent manner. ATP6V0C competed with Von Hippel-Lindau protein in HIF-1alpha binding by directly interacting with HIF-1alpha, which was stimulated by bafilomycin. In confocal images, ATP6V0C was normally present in the cytoplasm but was translocated in company with HIF-1alpha to the nucleus by bafilomycin. The N-terminal end (amino acids 1-16) of HIF-1alpha was identified as the ATP6V0C-interacting motif. These results suggest that ATP6V0C, a novel regulator of HIF-1alpha, mediates HIF-1alpha expression by bafilomycin.en
dc.language.isoenen
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics (ASPET)en
dc.subjectAmino Acid Motifsen
dc.subjectBinding, Competitiveen
dc.subjectEnzyme Inhibitors/*pharmacologyen
dc.subjectHumansen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectHypoxia-Inducible Factor 1, alpha Subunit/chemistry/*metabolismen
dc.subjectMacrolides/*pharmacologyen
dc.subjectVacuolar Proton-Translocating ATPases/antagonists & inhibitors/*metabolismen
dc.subjectVon Hippel-Lindau Tumor Suppressor Protein/*metabolismen
dc.titleATP6V0C competes with von Hippel-Lindau protein in hypoxia-inducible factor 1alpha (HIF-1alpha) binding and mediates HIF-1alpha expression by bafilomycin A1en
dc.typeArticleen
dc.contributor.AlternativeAuthor임지홍-
dc.contributor.AlternativeAuthor박종완-
dc.contributor.AlternativeAuthor김성준-
dc.contributor.AlternativeAuthor김명석-
dc.contributor.AlternativeAuthor박상기-
dc.contributor.AlternativeAuthor전양숙-
dc.identifier.doi10.1124/mol.106.030296-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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