Publications
Detailed Information
Chloride conductance is required for the protein kinase A and Rac1-dependent phosphorylation of moesin at Thr-558 by KCl in PC12 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Songhee | - |
dc.contributor.author | Kim, Sohee | - |
dc.contributor.author | Kim, Eunhee | - |
dc.contributor.author | Lee, Ji Eun | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.contributor.author | Juhnn, Yong-Sung | - |
dc.contributor.author | Kim, Yong Sik | - |
dc.contributor.author | Bae, Chang-Dae | - |
dc.contributor.author | Park, Joobae | - |
dc.date.accessioned | 2010-01-12T04:30:04Z | - |
dc.date.available | 2010-01-12T04:30:04Z | - |
dc.date.issued | 2005-01-07 | - |
dc.identifier.citation | J Biol Chem. 2005 Apr 1;280(13):12181-9. Epub 2005 Jan 5. | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15634677 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29646 | - |
dc.description.abstract | Moesin is a member of the ERM family, a family of cross-linkers between the plasma membrane and the actin cytoskeleton, which are known to be activated by phosphorylation. Previously, we reported the RhoA and Rho kinase-dependent phosphorylation of moesin at Thr-558 in hippocampal neuronal cells by glutamate. Here we studied the induction of moesin phosphorylation by KCl (60 mm) in PC12 cells. Moesin phosphorylation at Thr-558 was increased after 2 min of KCl treatment, peaked at 5 min, and returned to the basal level by 60 min. KCl also activated Rac1, but not RhoA, in PC12 cells, and KCl-induced moesin phosphorylation was suppressed in dominant negative Rac1 (N17 Rac1)-expressed cells. The inhibition of protein kinase A (PKA), known as an upstream kinase of Rac1, abolished Rac1 activation and moesin phosphorylation by KCl. Interestingly, the phosphorylation of moesin by KCl was independent of KCl-induced membrane depolarization and calcium influx but was dependent on KCl-induced chloride conductance. 60 mm KCl induced chloride conductance in PC12 cells, and pretreatment with Cl- channel blocker abolished Rac1 activation and moesin phosphorylation by KCl. These results suggest that the phosphorylation of moesin at Thr-558 in PC12 cells by KCl treatment is PKA- and Rac1-dependent and that KCl-induced chloride conductance is involved in the activation of this signaling system. | en |
dc.language.iso | en | en |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.subject | Animals | en |
dc.subject | Chloride Channels/*metabolism | en |
dc.subject | Chlorides/*metabolism | en |
dc.subject | Culture Media, Serum-Free/pharmacology | en |
dc.subject | Cyclic AMP-Dependent Protein Kinases/*metabolism | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Immunoblotting | en |
dc.subject | Microfilament Proteins/chemistry/*metabolism | en |
dc.subject | PC12 Cells | en |
dc.subject | Patch-Clamp Techniques | en |
dc.subject | Phosphorylation | en |
dc.subject | Plasmids/metabolism | en |
dc.subject | Potassium/chemistry | en |
dc.subject | Potassium Chloride/*metabolism | en |
dc.subject | Rats | en |
dc.subject | Signal Transduction | en |
dc.subject | Threonine/*metabolism | en |
dc.subject | Time Factors | en |
dc.subject | Transfection | en |
dc.subject | rac1 GTP-Binding Protein/*metabolism | en |
dc.subject | rhoA GTP-Binding Protein/metabolism | en |
dc.title | Chloride conductance is required for the protein kinase A and Rac1-dependent phosphorylation of moesin at Thr-558 by KCl in PC12 cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 전송희 | - |
dc.contributor.AlternativeAuthor | 김소희 | - |
dc.contributor.AlternativeAuthor | 김은희 | - |
dc.contributor.AlternativeAuthor | 이지은 | - |
dc.contributor.AlternativeAuthor | 김성준 | - |
dc.contributor.AlternativeAuthor | 전용성 | - |
dc.contributor.AlternativeAuthor | 김용식 | - |
dc.contributor.AlternativeAuthor | 배창대 | - |
dc.contributor.AlternativeAuthor | 박주배 | - |
dc.identifier.doi | 10.1074/jbc.M408253200 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.